Elucidation of mechanism for spontaneous molecular-recognition in lipid non-bilayer membranes

脂质非双层膜自发分子识别机制的阐明

基本信息

批准号：
15550122
负责人：
MATSUKI Hitoshi
金额：
$ 2.43万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15550122/
关键词：
Phospholipid Lipid bilayer membrane Phase transition Non-bilayer structure Interdigitated gel phase Hexagonal II phase High pressure Molecular recognition 相図コレステロール巨大ベシクル巨大単層ベシクル麻酔薬

项目摘要

In this study, we investigated the molecular recognition ability of lipid molecules in non-bilayer aggregates formed by phospholipids, that is, interdigitated gel and inverted hexagonal structures, by means of macroscopic methods such as differential scanning calorimetry and high-pressure light transmittance measurements and a microscopic method as a high-pressure fluorescent probe measurement. The obtained results are summarized as follows :1.The bilayer phase transitions for four kinds of N-methylated dipalmitoylphospatidylethanolamines with different-sized polar head groups under high pressure were examined. The stability and polymorphism of gel phases among the phospholipid bilayers were revealed.2.The barotropic phase transitions of asymmetric phosphatidylcholine bilayer membrane were observed. Interdigitation of asymmetric phosphatidylcholine was markedly different from those of symmetric ones.3.The fluorescence spectra of Prodan for the bilayer membranes of four kinds of diacylp … More hosphatidylcolines with varying acyl chain lengths were observed as a function of pressure. We speculated the location of the Prodan molecules in bilayer membranes in different phases from the shift of fluorescence spectra.4.The phase transition three kinds of phosphatidylcholine bilayer membranes in deuterium oxide and hydrogen oxide were examined. The substitution of hydrogen oxide by deuterium oxide produced a great effect on the transitions between gel phases and unstabilized the interdigitated gel phase.5.The fluorescence spectra of Prodan and Laurdan in bilayer membranes of phosphatidylcholine were observed as a function of pressure at constant temperature. In being formed the interdigitated gel phase, the Laurdan molecule was not squeezed out from the glycerol backbone region of phospholipid molecules because of the long acyl chain.6.The phase transition between the liquid crystal phase and the inverted hexagonal phase of dioleoylphosphatidylethanolamine bilayer membrane in aqueous NaCl solutions was investigated. We concluded that the transition can be regarded as the volume-driven transition for the reconstruction of molecular packing. Less

在这项研究中，我们研究了由磷脂形成的非双层聚集体中脂质分子的分子识别能力，即通过宏观扫描量表和高压测量型和高压测量值和高量型高量型高质量型方法，该方法是通过宏观扫描方法和抗六边形结构的相互作用和倒置的六边形结构。所获得的结果总结如下：1。对四种N-甲基化的二甲米型甲氧酰磷脂乙醇胺的双层相变，研究了高压下具有不同大小的极性头部的双层二酰磷脂。揭示了磷脂双层中凝胶相的稳定性和多态性。2。观察到不对称磷脂酰胆碱双层膜的正压相变。不对称磷脂酰胆碱的插图与对称的磷脂酰胆碱明显不同。3。prodan的荧光光谱对于四种二酰二酰基的双层膜的荧光光谱……较大的hosphidylcoline具有不同乙酰链链长的丙链酰链，从而观察到酰基链长度的变化。我们猜测，荧光光谱的偏移从不同相的双层膜中prodan分子的位置。用氧化氘取代氧化氢对凝胶相之间的过渡产生了很大的影响。在形成相互作用的凝胶相中，由于长长的酰基链，未从磷脂分子的甘油主链区域挤出Laurdan分子。6液晶相之间的相变和二核酰基甲基磷脂型甲基乙烷甲基甲基甲素的二甲基甲基甲基酯的六边形相位的相变。我们得出的结论是，该过渡可以视为分子堆积重建的体积驱动的转变。较少的