The Study on Proteinaceous Toxins from the Cnidarians.

刺胞动物蛋白质毒素的研究。

• 批准号: 15510172
• 负责人: NAGAI Hiroshi
• 金额: $ 2.3万
• 依托单位: Tokyo University of Marine Science and Technology (2004-2005)東京水産大学 (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15510172/
• 关键词: Chiropsalmus quadrigatus; protein toxin; cytotoxicity; hemolytic activity; Actineria villosa; fire coral; アカクラゲ; ミズクラゲ; タンパク質; 毒素; 致死活性; ウンバチイソギンチャク; PsTX-60B; 刺胞動物; AvTX-60A; MACPF

From the fiscal year 2003 to 2005, we have mainly studied on the isolation and chemical characterization of proteinaceous toxins obtained from Okinawan marine venomous animals. During these studies, we have found out the box jellyfish Chiropsalmus quadrigatus (habukurage in Japanese) has several proteinaceous toxins of which mode of action differs to each other. This result suggests that the stinging syndrome by C.quadrigatus is composed of multiple symptoms. The Okinawan sea anemone Actineria villosa causes severe cases of stinging. However, the causative toxins of the stinging by this species have not yet been known. Subsequently, we isolated the proteinaceous toxin which designated as A.viliosa toxin 60A (AvTX-60A, ca.60 kDa) as the major toxin. The cDNA encoding AvTX-60A was sequenced. The deduced amino acid sequence of AvTX-60A showed the MACPF domain. The hemolytic proteinaceous toxin has been successfully isolated from the venomous fire coral, Millepora sp. This toxin had an acidic isoelectric point and the molecular weight of around 100 kDa. This is the first reported case of the isolation of a high molecular weight (100 kDa) protein toxin as its active form from cnidarians.

从2003财年到2005财年，我们主要研究从冲绳海洋有毒动物中获得的蛋白质毒素的分离和化学表征。在这些研究中，我们发现箱形水母Chiropsalmus fourrigatus（日语为habukurage）含有多种蛋白质毒素，其作用方式各不相同。这一结果表明，C.quadrigatus 的蜇伤综合征是由多种症状组成的。冲绳海葵会导致严重的蜇伤。然而，目前尚不清楚该物种蜇人的致病毒素。随后，我们分离出蛋白质毒素，将其命名为A.viliosa毒素60A（AvTX-60A，ca.60 kDa）作为主要毒素。对编码 AvTX-60A 的 cDNA 进行了测序。 AvTX-60A的推导氨基酸序列显示MACPF结构域。溶血性蛋白质毒素已成功从有毒的火珊瑚（Millepora sp.）中分离出来。该毒素具有酸性等电点，分子量约为 100 kDa。这是首次报道从刺胞动物中分离出活性形式的高分子量（100 kDa）蛋白质毒素。

项目成果

A new membrane-attack complex/ perforin (MACPF) domain fatal toxin from the nematocyst venom of the Okinawan sea anemone Actineria villosa

冲绳海葵刺丝囊毒液中的一种新的膜攻击复合物/穿孔素（MACPF）结构域致命毒素

• 发表时间: 2004
• 期刊: Toxicon 43
• 作者: Naomasa Oshiro;Chiho Kobayashi;Setsuko Iwanaga;Masatoshi Nozaki;Michio Namikoshi;Jurg Spring;Hiroshi Nagai
• 通讯作者: Hiroshi Nagai

Michio Namikoshi, Rika Negishi, Hiroshi Nagai, Andrey Dmitrenok, Hisayoshi Kobayashi: "Three New Chlorine Containing Antibiotics from a Marine-derived Fungus Aspergillus ostianus Collected in Phonpei."Journal of Antibiotics. 56. 755-761 (2003)

Michio Namikoshi、Rika Negishi、Hiroshi Nagai、Andrey Dmitrenok、Hisayoshi Kobayashi：“从丰佩收集的海洋来源真菌 Aspergillus ostianus 中提取出三种新的含氯抗生素。”抗生素杂志。

Lissoclibadins 1-3, three new polysulfur alkaloids, from the ascidian Lissoclinum cf. badium

Lissoclibadins 1-3，三种新的多硫生物碱，来自海鞘类 Lissoclinum cf.

• 发表时间: 2005
• 期刊: Tetrahedron 61
• 作者: Hongwei Liu;Takeshi Fujiwara;Teruaki NishiKawa;Yuri Mishima;Hiroshi Nagai;Takeshi Shida;Kazuo Tachibana;Hisayoshi Kobayashi;Remy E.P.Mangindaan;Michio Namikoshi.
• 通讯作者: Michio Namikoshi.

wo New Manoalide Derivatives Isolated as Natural Products from a Marine Sponge Luffariella sp.Collected in Palau.

从帕劳收集的海洋海绵 Luffariella sp. 中分离出作为天然产物的新型 Manoalide 衍生物。

• 发表时间: 2004
• 期刊: Fisheries Science 70
• 作者: Michio Namikoshi;Shigeo Suzuki;Shiori Meguro;Hiroshi Nagai;Yoshio Koike;Akira Kitazawa;Hisayoshi Kobayashi;Taiko Oda;Junko Yamada.T
• 通讯作者: Junko Yamada.T

沖縄産ウンバチイソギンチャクの刺胞毒に関する研究.

冲绳日本海葵线虫囊毒素的研究.

• 发表时间: 2003
• 期刊: うみうし通信 38
• 作者: 大城直雅;永井宏史
• 通讯作者: 永井宏史