Dynamics of Synaptic Proteins in C.elegans

线虫突触蛋白的动力学

• 批准号: 15500208
• 负责人: KUROYANAGI Hidehito
• 金额: $ 2.37万
• 依托单位: Tokyo Medical & Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500208/
• 关键词: C.elegans; visualization; fluorescent protein; glutamate receptor; synapse; 経時観察

Long-term visualization of synaptic proteins and spine morphology in mammalian neurons revealed continual turnover of a fraction of synapse population in vitro and in vivo. Furthermore, recent analysis of mammalian synaptic proteins showed rapid alterations of protein compositions in synapses and their regulation by neuronal activity. Significance of continual alterations of synaptic structure and molecular compositions should be evaluated in a system where direct correlational analyses between changes of circuit properties and resulting behavioral alterations are possible. In C.elegans, full descriptions of the circuitry of all 302 neurons are available and specific sets of neurons and genes that control the sensory-motor coordination have been identified. Therefore, long-term visualization of synaptic proteins essential in the proper synaptic functions between neurons involved in the sensory-motor coordination would be an ideal model system to test the behavioral significance of syna … More pse remodeling.To this end, we focused on the glutamatergic synapses between polymodal sensory neuron ASH and interneurons of the locomotory control circuit (command neurons) of C.elegans. The glutamatergic transmission at ASH-command neuron synapses is essential for response to nose-touch. To visualize postsynaptic component, we utilized a fusion protein between ionotropic glutamate receptor GLR-1 and green fluorescent protein (GLR-1::GFP) and expressed this reporter gene either by an authentic glr-1 promoter or nmr-1 promoter that support expression of GLR-1 in a subset of interneurons. Images were obtained by a two-photon laser scanning microscope. Transgenic worms expressing GLR-1-GFP under the control of glr-1 promoter showed fluorescent clusters in both the nerve ring and the ventral nerve cord. Time-lapse imaging of short- and long-term remodeling of GLR-1::GFP clusters in the ventral nerve cord revealed both short-term structural stability and development-dependent remodeling. In transgenic worms expressing GLR-1::GFP under the control of nmr-1 promoter, a smaller number of fluorescent clusters were visualized within the nerve ring. Anatomical location and double labeling of ASH neurons with longer-wavelength fluorescent probes suggested that some of the GLR-1::GFP clusters corresponded to the synaptic junctions between ASH and command neurons. Less

哺乳动物神经元中合成蛋白和脊柱形态的长期可视化表明，体外和体内突触中的一小部分。此外，对哺乳动物合成蛋白的最新分析显示，突触中蛋白质组成的快速改变及其通过神经元活性调节。在系统中，应在电路特性变化和产生的行为改变之间进行直接相关分析的系统中，应评估合成结构和分子组成的连续改变的重要性。在C.元素中，所有302个神经元的电路的完整描述都可以使用，并且已经确定了控制感觉运动配位的特定神经元和基因。 Therefore, long-term visualization of synthetic proteins essential in the proper synaptic functions between neurons involved in the sensory-motor coordination would be an ideal model system to test the behavioral significance of Syna … More pse remodeling.To this end, we focused on the glutamatergic synapses between polymodal sensory neuron ASH and interneurons of the locomotory control circuit (command neurons) of C.elegans.灰分命令神经元突触的谷氨酸能传播对于对鼻触摸的反应至关重要。为了可视化突触后成分，我们利用了离子型谷氨酸能受体GLR-1和绿色荧光蛋白（GLR-1 :: GFP）之间的融合蛋白，并通过真实的GLR-1启动子或NMR-1启动子表达Glr-1在Interneyurons in Interneurons sepset of Neyurons in Interneurons中的表达表达。图像是通过两光子激光扫描显微镜获得的。在GLR-1启动子控制下表达GLR-1-GFP的转基因蠕虫显示神经环和腹神经绳的荧光簇。 GLR-1 :: GFP簇的短期和长期重塑的延时成像揭示了短期结构稳定性和发展依赖性重塑。在NMR-1启动子控制下表达GLR-1 :: GFP的转基因蠕虫中，在神经环内看到了较少数量的荧光簇。具有较长波长荧光问题的灰神经元的解剖位置和双重标记表明，某些GLR-1 :: GFP簇对应于ASH和命令神经元之间的突触连接。较少的

项目成果

Impaired cell cylce control of neuronal precursor cells in the neocortical primordium of presenilin-1-deficient mice.

presenilin-1 缺陷小鼠新皮质原基中神经元前体细胞的细胞周期控制受损。

• 发表时间: 2002
• 期刊: J Neurosci Res. 70
• 作者: Y.Kashimori;N.Suzuki;K.Fujita;T.Kamabra;Yuasa S et al.
• 通讯作者: Yuasa S et al.

Impaired cell cycle control of neuronal precursor cells in the neocortical primordium of presenilin-1-deficient mice.

presenilin-1 缺陷小鼠新皮质原基中神经元前体细胞的细胞周期控制受损。

• 发表时间: 2002
• 期刊: J Neurosci Res. 70
• 作者: Yuasa S;Nakajima M;Aizawa H;Sahara N;Koizumi K;Sakai T;Usami M;Kobayashi S;Kuroyanagi H;Mori H;Koseki H;Shirasawa T.
• 通讯作者: Shirasawa T.

Simultaneous observation of stably associated presynaptic varicosities and postsynaptic spines : morphological alterations of CA3-CA1 synapses in hippocampal slice cultures.

同时观察稳定相关的突触前静脉曲张和突触后棘：海马切片培养物中 CA3-CA1 突触的形态变化。

• 发表时间: 2005
• 期刊: Molecular and Cellular Neuroscience 28
• 作者: Umeda;T.;Ebihara;T.;S.Okabe
• 通讯作者: S.Okabe

キーワードで理解する転写イラストマップ(田村隆明編)

用关键词理解的抄写插图图（田村隆明编辑）

• 发表时间: 2004
• 作者: 黒柳秀人;萩原正敏
• 通讯作者: 萩原正敏