Analysis of spatio-temporarl mechanisms on outer plexiform layer in retina by the multichannel microprobe electrodes array chip

多通道微探针电极阵列芯片分析视网膜外丛状层时空机制

• 批准号: 15360218
• 负责人: USUI Shiro
• 金额: $ 5.06万
• 依托单位: RIKEN
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15360218/
• 关键词: retina; multichannel recording; mathematical models; electroretinogram(ERG); bipolce cell; horizontal cell; microprobe electrode; マルチチャネル記録; 網膜電位図(ERG); 光応答計測; マイクロプローブ

We have developed multichannel microprobe electrode array chips for recording neural responses to light from retina, and mathematical model of retinal neurons to analyze retinal mechanisms involved in the recorded light-induced responses.Prototypes of the microprobe electrode array(MEA) chip have been fabricated on Si(111) substrates by selective Vapor-Liquid-Solid(VLS) growth method. The prototype MEA could detect light responses whose characteristics to a white flash and sensitivities to monochromatic stimulus were consistent with photopic electoretinogram(ERG). We also demonstrated that the changes of localized ERG responses were obtained with stimulus of slit light by the MEA.In order to analyze inner retinal mechanisms involved in the measured ERG responses, we have developed models of retinal neuron and the retinal neural circuit by physiological engineering technique. The models of horizontal cell and bipolar cells which is closely related to form b-wave in ERG responses were described mathematically by their ionic mechanisms. It is allow us to analyze the relationship between light responses and the ionic mechanisms in their neuron.These results suggest that the developed MEA chip is applicable to retinal neural recordings as a new measurement tool, and the models of retinal neuron would play a role to understand visual information preprocessing of retina.

我们已经开发了用于记录视网膜光的神经反应的多通道微探针阵列芯片，以及视网膜神经元的数学模型，以分析与记录的光诱导的响应有关的视网膜机制。在SI si（111）sisseledive vaprorivive vaptrorivivive vaptroricive vaptroricive（MEA）芯片的绘制中，对光型电极阵列（MEA）芯片进行了构建。原型MEA可以检测到对白色闪光的特征和对单色刺激的敏感性的光反应，与光波选择性图（ERG）一致。我们还证明，通过MEA刺激SLIT光的局部ERG反应的变化。为了分析测量ERG反应所涉及的内部视网膜机制，我们通过生理工程技术开发了视网膜神经元和视网膜神经回路的模型。与ERG反应中B波密切相关的水平细胞和双极细胞模型通过其离子机制描述了数学上。它使我们能够分析其神经元中光反应与离子机制之间的关系。这些结果表明，开发的MEA芯片适用于视网膜神经记录作为一种新的测量工具，视网膜神经元的模型将起作用，可以理解视网膜的视觉信息预处理。

项目成果

Nakauchi S, Ishihara A, Usui S: "Fundamental research on visual information processing"Proceedings of the Second Symposium of Intelligent Human Sensing. 47-50 (2004)

中内S、石原A、臼井S：《视觉信息处理的基础研究》第二届人类智能感知研讨会论文集。

Selective vapor-liquid-solid epitaxial growth of micro-SI probe electrode arrays with on-chip MOSFETs on Si(111) substrates

• DOI: 10.1109/ted.2003.822473
• 发表时间: 2004-03-01
• 期刊: IEEE TRANSACTIONS ON ELECTRON DEVICES
• 影响因子: 3.1
• 作者: Kawano, T;Kato, Y;Ishida, M
• 通讯作者: Ishida, M

Kawano T, Takao H, Sawada K, Ishida M: "Multichannel 5×5-site 3-dimensional Si micro-probe electrode array for neural activity recording"Journal of Applied Physics. 42. 2473-2477 (2003)

Kawano T、Takao H、Sawada K、Ishida M：“用于神经活动记录的多通道 5×5 位点 3 维硅微探针电极阵列”应用物理学杂志 42. 2473-2477 (2003)。

針本哲弘, 石原彰人, 河野剛士, 石田誠, 臼井支朗: "ヤイクロプローブ電極アレイによる網膜光応答計測"電子情報通信学会技術研究報告. 103・732. 59-64 (2004)

Tetsuhiro Harimoto、Akito Ishihara、Takeshi Kono、Makoto Ishida、Shiro Usui：“使用环探针电极阵列进行视网膜光响应测量”IEICE 技术报告 103・732 (2004)。

マイクロプローブ電極アレイによる網膜光応答計測

使用微探针电极阵列测量视网膜光反应

• 发表时间: 2004
• 期刊: 電子情報通信学会技術研究報告 103
• 作者: 針本;石原;河野;石田;臼井
• 通讯作者: 臼井