Analysis of spatio-temporal light responses of retinal cells using a new multi-electrode array on integrated circuit

使用集成电路上的新型多电极阵列分析视网膜细胞的时空光响应

• 批准号: 13559003
• 负责人: USUI Shiro
• 金额: $ 3.01万
• 依托单位: Toyohashi University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13559003/
• 关键词: retina; multi-electrode array; neuron; modeling simulation; neural signal recording; synapse; bipolar cell; ganglion cell; 集積型微小電極アレイ; 網膜神経節細胞; 網膜双極細胞; シナプス終末

The purpose of our research is the elucidation of neural circuit mechanisms concerning with spatio-temporal visual processing in retina by the approach of both electrophysiological experiment using multi-electrode and modeling simulation.We developed the new multi-microelectrode array which is suitable to record light responses from retinal ganglion cell. The array was developed using selective vapor-liquid-sold (VLS) growth method and integrated circuit (IC) process on Si(111) substrate. The length and diameter of probes on the array was controlled in a few micrometers. We also established the electrophysiological recording system using the array based on the ordinary system.In the modeling approach, we developed mathematical model of synaptic mechanisms between bipolar cell and ganglion cell. The model described intracellular transmission mechanisms in synapse of bipolar cell based on their physiological evidence, and well reproduced the I-V relationship and the capacitance jump responses to pulses with various durations. The model would allow us to construct network model from photoreceptor to ganglion cell, and to analyze neural mechanisms in direct pathway of visual information in retina.

我们研究的目的是通过使用多电极和建模仿真的电生理实验的接近来阐明视网膜中与时空视觉处理有关的神经回路机制。我们开发了新的多微米电极阵列，可记录视网膜神经节细胞的光反应。使用选择性蒸气液（VLS）生长法和Si（111）底物上的集成电路（IC）过程开发阵列。阵列上的探针的长度和直径在几微米中控制。我们还使用基于普通系统的阵列建立了电生理记录系统。在建模方法中，我们开发了双极细胞和神经节细胞之间突触机制的数学模型。该模型根据其生理证据描述了双极细胞突触中的细胞内传播机制，并很好地再现了I-V的关系以及对各种持续时间的脉冲的电容跳跃反应。该模型将使我们能够构建从光感受器到神经节细胞的网络模型，并分析视网膜视觉信息直接途径的神经机制。

项目成果

河北友克, 石原彰人, 臼井支朗, 立花政夫: "網膜双極細胞シナプス終末の二相性放出現象を再現するモデル"平成13年度電気関係学会東海支部連合大会講演論文集. 346. 11-11 (2001)

Tomokatsu Kawakita、Akito Ishihara、Shiro Usui、Masao Tachibana：“再现视网膜双极细胞突触末端双相释放现象的模型”2001 年电气工程学会东海分会会议记录 346. 11-11 (2001)。 ）

Kawano, T., Kato, Y., Futagawa, M., Tani, R., Takao, H., Sawada, K., Ishida, M: "Fabrication and Properties of Ultra Small Si Wire Arrays by Vapor-Liquid-Solid Growth with Circuits"Sensors and Actuators A. 97-98. 709-715 (2002)

Kawano, T.、Kato, Y.、Futakawa, M.、Tani, R.、Takao, H.、Sawada, K.、Ishida, M：“气-液-固超小型硅线阵列的制造和性能

Kato, Y., Kawano, T., Ito, Y., Ashiki, M., Takao, H., Sawada, K., Ishida, M: "Signal Conditioning CMOS Circuit Integrated on Si (111) for Neural Activity Image Recording"IEEJ The 19th Sensor Symposium. 421-424 (2002)

Kato, Y.、Kawano, T.、Ito, Y.、Ashiki, M.、Takao, H.、Sawada, K.、Ishida, M：“用于神经活动图像记录的信号调节 CMOS 电路集成在 Si (111) 上

Kawano, T., Kato, Y., Futagawa, M., Tani, R., Takao, H., Sawada, K., Ishida, M: "Vapor-liquid-solid Graon Si Probe Array Chip for Penetrating and Recording in the Nervous System"IEEJ The 19th Sensor Symposium. 487-490 (2002)

Kawano, T.、Kato, Y.、Futakawa, M.、Tani, R.、Takao, H.、Sawada, K.、Ishida, M：“用于穿透和记录的气-液-固 Graon Si 探针阵列芯片

T.Kawano, Y.Kato, M.Futagawa, H.Takao, K.Sawada, M.Ishida: "Fabrication and Properties of Ultra Small Si Wire Arrays by Vapor-Liquid-Solid Growth with Circuits"Digest of Technical Papers of The 11th International Conference on Solid & State Sensors and Ac

T.Kawano、Y.Kato、M.Futakawa、H.Takao、K.Sawada、M.Ishida：“通过气-液-固生长电路实现超小型硅线阵列的制造和性能”技术论文摘要