Interaction of three dimensional network of the feeding regulation neurons and steroid hormones

摄食调节神经元三维网络与类固醇激素的相互作用

基本信息

批准号：
14580772
负责人：
OZAWA Hitoshi
金额：
$ 2.62万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

It has been reported that there are two orexin (from the Greek word for appetite, orexis) peptides derived from pre-pro-orexin, designated as orexin A and B. Orexin A is a 33-residue peptite with two intramolecular disulfide bonds in the N-terminal region, and orexin B is a linear 28-residue peptide. These peptides, encoded by a single mRNA transcript, has a 46% amino acid sequence identity (Sakurai T et al. 1998). Their effects are mediated by receptors, orexin 1 and 2 receptor. Orexin-A has ha similar affinity for both receptors, whereas orexin-B has a higher affinity for the orexin 2 receptor subtype.It has been also reported that orexin-A and -B mRNA and their cell bodies were mainly detedted in the hypothalamic area, particularly in the lateral hypothalamic area and the perifornical nucleus. Recently, it has been reported that the distribution of immunoreactive fibers overlaps with the luteinizing hormone-releasing hormone(LHRH) neuronal system in the septopreoptic area and the ar … More cuate nucleus median eminence region, suggesting that orexins are involved with the sex hormone secretion. However, the sex difference of the expression of orexin-A and -B has not been elucidated. In the project, we investigated the sex difference of the distribution pattern of the feeding related neurons, particularly orexin neuorns and the effect of estrogen for the expression of orexin peptides in the male, female and ovariectomized (OVX) rats.Orexin neurons are distributed in the perifornical and lateral hypothalamic area, a region classically implicated in the control of feeding behavior, and project their axons within the hypothalamus and throughout the central nervous system to be involved in the control of feeding. In the project, we investigated the sexual difference of orexin neurons using specific orexin-A and orexin-B antibodies. The density of both orexin-A and orexin-B immunopositive neurons in male was preferentially higher than those in female rats. On the other hand, ovariectomy in female rats induced the change to the male pattern. Colocalization of orexin and estrogen receptor was not observed. These results suggested that orexin neurons were indirectly regulated by estrogen through the other estrogen dependent neurons. Less

据报道，有两种由前食欲素原衍生而来的食欲素（来自希腊语，食欲的意思，orexis）肽，分别命名为食欲素A和B。食欲素A是一种33个残基的肽，在肽段中具有两个分子内二硫键。 N 末端区域，食欲素 B 是一种线性 28 个残基肽，这些肽由单个 mRNA 转录物编码，具有 46% 的氨基酸。它们的作用是由受体介导的，食欲素 1 和 2 受体对两种受体具有相似的亲和力，而食欲素 2 受体亚型则具有更高的亲和力。也有报道，orexin-A和-B mRNA及其细胞体主要在下丘脑区检测，特别是下丘脑外侧区和下丘脑外侧区。最近，有报道称，免疫反应性纤维的分布与视隔前区和角核正中隆起区的黄体生成素释放激素（LHRH）神经元系统重叠，表明食欲素与穹窿周围核的分布有关。然而，orexin-A和-B表达的性别差异尚未阐明。在该项目中，我们调查了性别差异的分布模式。摄食相关神经元，特别是食欲素神经元，以及雌激素对雄性、雌性和卵巢切除（OVX）大鼠中食欲素肽表达的影响。食欲素神经元分布在穹窿周围和外侧下丘脑区域，该区域通常与对照有关摄食行为，并将它们的轴突投射到下丘脑和整个中枢神经系统中，以参与摄食的控制。在该项目中，我们研究了性别差异。使用特异性食欲素-A和食欲素-B抗体的食欲素神经元雄性大鼠中食欲素-A和食欲素-B免疫阳性神经元的密度优先高于雌性大鼠。没有观察到食欲素和雌激素受体的共定位，这些结果表明食欲素神经元通过其他雌激素依赖性神经元间接受到雌激素的调节。

项目成果

期刊论文数量（48）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Steroid efects on secretion from subsets of lactotrophs : role of folliculo-stellate cells and annexin 1.

类固醇对泌乳素亚群分泌的影响：滤泡星状细胞和膜联蛋白 1 的作用。

DOI：
发表时间：
2002
期刊：
Arch Physiol Biochem 110
影响因子：
0
作者：
Morris J.F.;Christian H.C.;Chapman L.P.;Epton M.J.;Buckingham J.C.;Ozawa H.;Nishi M.;Kawata M.
通讯作者：
Kawata M.

Exocytosis sensitivity to growth hormone-releasing hormone in subsets of GH cells in rats under different corticosterone condition. Ultrastructural study using microwave irradiation for fixation

不同皮质酮条件下大鼠 GH 细胞亚群的胞吐作用对生长激素释放激素的敏感性。