Morphological analyses about origins, neurochemical substances and target-specificily of neurons coming to the subfornical organ

关于穹窿下器官神经元的起源、神经化学物质和目标特异性的形态学分析

基本信息

批准号：
14580771
负责人：
KAWANO Hitoshi
金额：
$ 1.92万
依托单位：
Saga University (2004)佐賀医科大学 (2002-2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Origins, neurochemical substances and target-specificity of neurons coming to the subfornical organ(SFO) were investigated using anterograde and retrograde tracing technique combined with immunohistochemistry. Distribution and target elements of nerve terminals containing several neurochemical substances were investigated for the basis of the study. There were aminergic, cholinergic and peptidergic nerve terminals in the SFO. Serotonin-,Met-enkephalin-8(mE8)-,galanin(GAL)-,neurotensin- and luteinizing hormone-releasing hormone-immunoreactive nerve terminals made synapses with cell bodies and dendrites, and contacted closely with vessels and glial cells in the SFO. Origins of mE8-immunoreactive projections were the anterodorsal and anteroventral periventricular nuclei, preoptic and hypothalamic periventricular regions, median preoptic nucleus, hypothalamic dorsomedial and arcuate nuclei, medial parabrachial nucleus and solitary nucleus ; GAL-immunoreactive projections were the medial preoptic nucleus, medial parvocellular paraventricular nucleus, hypothalamic periventricular regions, hypothalamic dorsomedial and arcuate nuclei ; bornbesin-immunoreactive projections were the medial parvocellular paraventricular nucleus and rostral ventrolateral medulla ; neuropeptide Y-immunoreactive projections were the hypothalamic arcuate nucleus, solitary nucleus and ventrolateral medulla ; cholinergic projections were the medial septal nucleus and diagonal band. Projections to the SFO from the median preoptic nucleus were huge. They made synapses with cell bodies and dendrites, and contacted closely with vessels and glial cells.It is very interesting to clear the relationship between SFO neurons projecting to one of the main targets, the paraventricular nucleus and nerve terminals coming from main origins, such as the median preoptic nucleus, lateral parabrachial nucleus and raphe nuclei. This is the matter to be solved.

使用同类和逆行跟踪技术与免疫组织化学相结合，研究了对属骨架器官（SFO）的神经化学物质和靶标特异性。研究了包含几种神经化学物质的神经末端的分布和靶元素以进行研究。 SFO中有氨基能，胆碱能和肽性神经末端。 5-羟色胺，含有脑蛋白-8（ME8） - ，甘丙蛋白（GAL） - ，神经素 - 释放激素释放激素 - 免疫反应神经反应性神经末端与细胞体和树突的突触，并与血管和胶质细胞中的SCFO紧密接触。。 ME8免疫反应性投射的起源是前和腹膜前脑周围核，牙本质前和下丘脑周围室性核，中值前核，下丘脑背侧核和弧形核，内侧副核核和内侧副核核和独立核核和孤立核。 GAL免疫反应性投影是内侧前核，内侧脊髓脊髓核，下丘脑脑室周围区域，下丘脑背侧和弧形核； BONTBESIN-免疫反应性预测是内侧旁侧室室核和thostral腹外侧髓质。神经肽y免疫反应性突显是下丘脑弓形核，孤立核和腹外侧髓质。胆碱能投射是内侧间隔核和对角线带。从中值前核向SFO投影非常巨大。他们与细胞体和树突形成了突触，并与血管和神经胶质细胞紧密接触。清除投射到主要靶标的SFO神经元之间的关系非常有趣上核前核，外侧副核核和raphe核。这是要解决的问题。