GENETICAL RESEARCH OF ORIGIN OF ASIAN SUSPOPULATIONS IN VIETNAM

越南亚洲人群起源的遗传学研究

• 批准号: 14405028
• 负责人: ISHIGURO Naotaka
• 金额: $ 7.49万
• 依托单位: GIFU UNIVERSITY (2004)Obihiro University of Agriculture and Veterinary Medicine (2002-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14405028/
• 关键词: wild boar; pig; Vietnam; Genetic analysis; phylogenetic analysis; Wild animal; Domestication

The purpose of this research is to characterize whether the origin of East Asian wild boar and domestic pigs is located in Vietnam by morphological and genetic analyses. For three years from 2002 to 2004, we visited several local villages to collect samples from wild boar and native pigs in North, West and middle areas of Vietnam. About morphological analysis, Sus bones collected from villages were measured, whereas, genetic analyses were conducted to collect heir and meat from native pigs, isolate a total of DNA from the samples and amplify mitochondrial DNA from DNA samples. A total of 248 DNA samples were sequenced and phylogenetic analized. The following results were obtained.1)Ryukyu wild boar was genetically related to Vietnamese wild boar. The present results supported the hypothesis that Ryukyu wild boars are a unique species established on isolated Ryukyu Islands.2)Vietnamese wild boars and domestic pigs possess remarkable genetic diversity. Vietnamese pigs, including wild and domestic pigs may share a common ancestor with other East Asian pigs.3)Morphological and genetic analyses of Vietnamese pigs will provide important information about the history of domestication pig in East Asian, because several pigs with different size or food habit are raising.A part of this research has been published in a paper, the other parts are now prepared in the papers.

这项研究的目的是表征东亚野猪和家养猪的起源是否通过形态和遗传分析位于越南。从2002年到2004年，我们参观了几个当地村庄，从越南北部，西和中部地区收集野猪和本地猪的样品。关于形态学分析，测量了从村庄收集的SUS骨骼，而进行了遗传分析以从天然猪中收集继承人和肉，从样品中分离了总共DNA，并从DNA样品中扩增了线粒体DNA。总共测序248个DNA样品并进行系统发育分析。获得以下结果。1）Ryukyu野猪在遗传上与越南野猪相关。目前的结果支持这样的假设：龙京野猪是在孤立的ryukyu岛上建立的独特物种。2）越南野猪和家猪具有显着的遗传多样性。包括野猪在内的越南猪可能与其他东亚猪共同祖先。3）越南猪的形态学和遗传分析将提供有关东亚猪毒猪历史的重要信息，因为几只具有不同大小或食物习惯的猪的养猪正在饲养。

项目成果

A genetic method to distinguish crossbred Inobuta from Japanese wild boars.

区分杂交猪和日本野猪的遗传方法。

• 发表时间: 2002
• 期刊: Zoological Science 19
• 作者: Ropert-Coudert Y;Kato A;Naito Y et al.;Naotaka Ishiguro
• 通讯作者: Naotaka Ishiguro

Genetic distribution map and origin ofJapanese wild boar. Natural history of animal geography (Masuda, R., Abe, N.eds)

日本野猪遗传分布图及起源.

• 发表时间: 2005
• 作者: Naotaka Ishiguro;Takuma Watanobe
• 通讯作者: Takuma Watanobe

Ancient DNA reveals genetic lineage of Sits scrofa, among archaeological sites in Japan.

日本考古遗址中的古代 DNA 揭示了 Sits scrofa 的遗传谱系。

• 发表时间: 2002
• 期刊: Anthropological Science 110
• 作者: Yasuko Morii;Naotaka Ishiguro;Takuma Watanobe;Masuo Nakano;Hitomi Hongo;Akira Matsui;Toyohikro Nishimoto
• 通讯作者: Toyohikro Nishimoto

Prehistoric Sado Island populations of Sus scrofa distinguished from contemporary Japanese wild boar by ancient mitochondrial DNA.

史前佐渡岛的野猪种群通过古老的线粒体 DNA 与当代日本野猪区分开来。

• 发表时间: 2004
• 期刊: Zoological Science 21
• 作者: Takuma Watanobe;Naotaka Ishiguro;Masuo Nakano;Akira Matsui;Hitomi Hongo;Kiyomi Yamazaki;Osamu Takahashi.
• 通讯作者: Osamu Takahashi.

Takuma Watanobe: "Phylogeography and population structure of the Japanese wild boar Sus Scrofa leucomystax : mitochondrial DNA variation"Zoological Science. 20. (2003)

Takuma Watanobe：“日本野猪 Sus Scrofa leucomystax 的系统发育地理学和种群结构：线粒体 DNA 变异”动物学。