STUDY ON THE NERVE-IMMUNE SYSTEMS AND CELLULAR FACTORS INFLUENCE TO THE PATHOGENICTTY AND DISTRIBUTION OF PRP^<Sc>IN ANIMAL PRION DISEASES

动物朊病毒疾病中神经免疫系统和细胞因素对PRP^<Sc>致病性和分布影响的研究

• 批准号: 17380180
• 负责人: ISHIGURO Naotaka
• 金额: $ 9.07万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380180/
• 关键词: Prion; Immunology; Nerve; Transport; Mouse; BSE; Scrapie; Ileum; 体内伝播; 回腸遠位部

The pathogenesis of animal prion diseases is influenced by prion strain, genetic factors, natural immunology and several cellular factors in the affected hosts. In this study, genetic factors, nerve-immune system and uptake of PrP^<Sc> by macrophages were mainly studied. The following results were obtained.1. Polymorpbisms of PrP gene: Polymorphisms of the 863 cattle, 118 caprine and 136 sika dear (Cervus Nippon) PrP genes in Japan were examined. Several single-nucleotide polymorphisms (SNPs) were found in cattle and sika dear, but not find any amino acid substitutions. Two polymorphisms associated with the resistance to scrapie were found in 118 caprine.2. Bovine and sheep nerve systems: To determine the location, namber and segmental distribution of sympathetic, parasympathetic and sensory neurons innervating the ileum in cattle and sheep, tracer experiments with horseradish peroxidase were conducted. The labeled neurons were detected in the celiac and cranial mesenteric ganglion complex in both cattle and sheep. Only a few labeled neurons existed in the caudal mesenteric ganglion and the paravertebral ganglia.3. Uptake PrP^<Sc> by immune cells: To clarify the uptake the PrP^<Sc> by immune cells, the involvement of bovine macrophage in the early stage of prion infection was studied. Brain homogenates of scrapie and BSE were degraded sequentially in bovine macrophage and bovine peripheral immune cells.A part of this research has been published in the papers, the other parts are now prepared in the papers.

动物prion疾病的发病机理受影响宿主中的遗传因素，自然免疫学和几个细胞因子的影响。在这项研究中，主要研究了遗传因素，神经免疫系统和对PRP^<SC的吸收。获得以下结果1。 PRP基因的多态性：日本的863头牛，118个Caprine和136 Sika Dear（Cervus nippon）PRP基因的多态性。亲爱的牛和西卡中发现了几种单核苷酸多态性（SNP），但找不到任何氨基酸取代。在118 caprine中发现了两种与crapi抗性有关的多态性。2。牛和绵羊神经系统：为了确定交感神经，副交感神经和感觉神经元的位置，namber和节段分布，对牛和绵羊的回肠支配，进行了对辣根过氧化物酶的示踪剂实验。在牛和绵羊的腹腔和颅肠神经节复合物中检测到标记的神经元。尾肠神经节和副神经节中只有少数标记的神经元。3。免疫细胞的吸收PRP^<sc>：为了阐明免疫细胞的摄取PRP^<sc>，研究了牛巨噬细胞在prion感染的早期阶段的参与。在牛巨噬细胞和牛外周免疫细胞中依次降解了碎屑和BSE的脑匀浆。这项研究的一部分已在论文中发表，其他部分现在在论文中制备了。

项目成果

Characterization of anti-chiken PrP antibodies

抗鸡 PrP 抗体的表征

• 发表时间: 2006
• 作者: Naotaka;Ishiguro;Yasuo;Inoshima;Yukiko;Sassa;Shinichi;Nakashima
• 通讯作者: Nakashima

Uptake system in bovine ileum considering prion infections

考虑朊病毒感染的牛回肠摄取系统

• 发表时间: 2007
• 作者: Lwine;Sein;Yukiko;Sassa;Naotaka;Ishiguro;Yasuo;Inoshima;Hiroshi;Ueno;Yasushige Ohmori
• 通讯作者: Yasushige Ohmori

ウシ回腸での物質の取り込み機構

牛回肠物质摄取机制

• 发表时间: 2008
• 作者: 石黒直隆;ら
• 通讯作者: ら

ニワトリ正常プリオン蛋白質に対する抗体の作成とその性状解析

抗正常鸡朊病毒蛋白抗体的制备及其特性分析

• 发表时间: 2006
• 作者: 石黒直隆;ら
• 通讯作者: ら

ウシとマウスの免疫系細胞の異常型プリオン蛋白質の取り込みと分解能

牛和小鼠免疫系统细胞对异常朊病毒蛋白的摄取和解析

• 发表时间: 2006
• 作者: 佐々悠木子;ら
• 通讯作者: ら