Experimental Research on Efficacy of Sequential Liver and Small intestinal transplantation

序贯肝小肠移植疗效的实验研究

• 批准号: 13470238
• 负责人: TAGUCHI Tomoaki
• 金额: $ 9.34万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470238/
• 关键词: Liver transplantation; Small bowel transplanation; Living donor; Sequential transplantation; Monitoring rejection; Tolerance; Motility; Rejection; 急性グラフト不全

Liver transplantation has been reported to induce donor specific tolerance. After tolerance induction by living related liver transplantation, small intestinal graft from the same donor can be accepted without immunosuppression.In the first year and second year, operation technique of living-relatied small intestinal plantation, using porcine model, was established. Ileal graft about 3 m were harvested with vascular pedicle, using two pigs, white pig and black pig. Cutting site of each superior mesenteric vessel was just distal to the most distal colonic branch. Then intestinal grafts were exchanged each other and graft artery was anastosed to Aorta and graft vein was anastomosed to IVC by end-to-side fashion.In the third year, graft motility was monitored by strain gage (S-G) force transducer. S-G transducer was fixed on the serosa. As a result, three parameters of MMC, duration, amplitude, and interval, were impaired in rejected grafts, while, MMC propagated throughout the graft and three parameters were normal in functional grafts. We conclude that he S-G method may afford real-time monitoring of transplanted intestinal motility that can be useful to detect rejection after small intestinal transplantation.In the force year, histological finding was compared with motility study. Histological mild rejection can be detected by S-G method. Combined sequential liver and small intestinal transplantation were succeeded in rat model, however porcine model has not been established yet.

据报道，肝移植可诱导供体特异性耐受。通过活体肝移植诱导耐受后，可以在不进行免疫抑制的情况下接受同一供者的小肠移植物。第一年和第二年，建立了猪模型的活体小肠移植手术技术。使用白猪和黑猪两头猪，收获带血管蒂的回肠移植物约3m。每条肠系膜上血管的切割部位正好位于最远端结肠分支的远端。然后将移植肠相互交换，移植动脉与主动脉吻合，移植静脉与下腔静脉端侧吻合。第三年，用应变计（S-G）力传感器监测移植物的运动。 S-G传感器固定在浆膜上。结果，MMC 的三个参数（持续时间、幅度和间隔）在排斥的移植物中受损，而 MMC 在整个移植物中传播，并且在功能性移植物中的三个参数正常。我们的结论是，S-G方法可以实时监测移植的肠道运动，有助于检测小肠移植后的排斥反应。在今年，将组织学发现与运动研究进行了比较。 S-G法可检测组织学轻度排斥反应。联合序贯肝小肠移植在大鼠模型中取得了成功，但猪模型尚未建立。

项目成果

Ogita K, Suita S, Taguchi T, Yamanouchi T, et al.: "Roles of nucleosides and nucleotide mixture in small bowel transplantation"Nutrition. 18. 338-342 (2002)

Ogita K、Suita S、Taguchi T、Yamanouchi T 等人：“核苷和核苷酸混合物在小肠移植中的作用”营养。

Fujita Y, Satoh S, Nakayama H, Taguchi T, Suita S, Nakano H: "In utero evaluation and the long-term prognosis of living infants with cystic hygroma"Fetal Diagnosis & Therapy. 16. 402-406 (2001)

Fujita Y、Satoh S、Nakayama H、Taguchi T、Suita S、Nakano H：“患有囊性水瘤的活婴儿的子宫内评估和长期预后”胎儿诊断

Ogita K, Suita S, Taguchi T, Yamamnouchi T, Masumoto K, Nakao M: "Benefit of nucleosides and uncleotide mixture in small bowel transplantation"Transplant Proc.. 34. 1027 (2002)

Ogita K、Suita S、Taguchi T、Yamamnouchi T、Masumoto K、Nakao M：“核苷和叔核苷酸混合物在小肠移植中的益处”Transplant Proc.. 34. 1027 (2002)

Taguchi T, Shima Y, Nakao M, Fujii Y, Suita S, et al.: "Cytokine response after segmental small intestinal transplantation : comparison of jejunal and ileal grafts"Transplant Proc.. 34. 1052 (2002)

Taguchi T、Shima Y、Nakao M、Fujii Y、Suita S 等：“节段性小肠移植后的细胞因子反应：空肠和回肠移植物的比较”Transplant Proc. 34. 1052 (2002)

Ogita K, Takada N, Taguchi T, Suita S, et al.: "Renal tubular acidosis secondary to FK506 in living donor liver transplantation : A case report"Asian J Surg. 26. 218-220 (2003)

Ogita K、Takada N、Taguchi T、Suita S 等人：“活体肝移植中 FK506 继发的肾小管性酸中毒：病例报告”Asian J Surg。