Assessment of graft viability after cold preservation in small intestinal transplantation.

小肠移植冷保存后移植物活力的评估。

基本信息

批准号：
04670785
负责人：
TAGUCHI Tomoaki
金额：
$ 1.34万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The precise assessment of graft viability is thought to be important, however there are still a few methods which remain controversial. We investigated the method for the assessment of small bowel graft viability, by means of physiological properties, energy metabolism, tissue blood flow, and the histological findings.Syngeneic heterotopic transplantation was performed using Lewisrats, after a cold preservation of a jejunal graft with UW solution for 6, 12, 24, or 48hr. Graft samples were taken before transplantation for physiological study, and at harvesting, at the end of cold storage, before and 30 min after reperfusion for histology and for the measurement of adenine nucleotides (HPLC method). The tissue blood flow of the graft was recorded using a laserdoppler flowmeter at reperfusion.The positive rates of samples showing both the biphasic nervous activities (excitation and inhibition) and the spontaneous rhythmic activity was 100%, 88%, and 25% in 0, 24, and 48 hr preservation, respectively. The level of ATP after reperfusion, which recovered to more than 30% of that at harvesting within 24 hr preservation groups but remained at less than 30% in the 48 hr group. The blood flow level at 5 or 30 min of reperfusion was significantly lower in the 48 hr group than that in the other groups. Histological damage after reperfusion, which extended deep into the crypt layr in the 48 hr group, but was confinded within villi in the other groups, correlated well with the results of physiology, energy metabolism, and tissu blood folw.The physiological property before transplantation, and the tissue ATP level and blood flow after reperfusion are considered to be useful parameters for assessment of small bowel graft viability. These results are correlated with the recipient survival which we had previously reported.

人们认为对移植生存能力的精确评估很重要，但是仍然有一些有争议的方法。我们研究了通过生理特性，能量代谢，组织血流和组织学发现评估小肠移植活力的方法。使用刘易斯拉斯（Lewisrats 6、12、24或48小时。移植前进行接枝样品进行生理研究，并在冷藏结束时，在收获后，再灌注后30分钟以进行组织学和测量腺嘌呤核苷酸（HPLC方法）。在再灌注时使用LaserDoppler流量计记录移植物的组织血流。样品的正速率均显示了双相神经活动（激发和抑制），并且自发性节奏活性为100％，88％，在0，25％，在0，25％。 24和48小时保存。再灌注后的ATP水平，在24小时保存组内收获时恢复了30％以上，但在48个小时组中的收获水平仍不到30％。在48小时组中，再灌注5或30分钟时的血流水平明显低于其他组。再灌注后的组织学损害，在48小时组中深处延伸到Crypt Layr中，但在其他组的Villi中被牢记，与生理学，能量代谢和Tissu Boldw的结果很好地相关。再灌注后的组织ATP水平和血流被认为是评估小肠移植活力的有用参数。这些结果与我们先前报道的接受者生存有关。