Ontogenetic and Phylogenelic anaylses of factors affecting the developmental process in crine concentrating mechanism

影响分泌物浓缩机制发育过程因素的个体发生和系统发育分析

基本信息

批准号：
13470208
负责人：
KONDO Yoshiaki
金额：
$ 9.34万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

In our previous investigation, we demonstrated that the neonatal renal medulla is transformed from 'avian-type' to 'mammalian-type' organization of the renal tubular functions. The transformations include the disappearance of active NaCl reabsorption and emergence of large passive NaCl reabsorption in the thin ascending limb of Henle's loop, and appearance of water permeability via AQP-1 in the thin descending limb.To elucidate the mechanism of transformation in the urine concentrating system of the renal medulla, we investigated the effect of elongation of the pregnancy period, thereby examining the influence of the birth on the transformational change in the system. The prolongation of pregnancy was achieved by two methods. One method is the vaglnal ligation on the day 17 during pregnancy. Another method is the administration of progesterone to the pregnant rats. Both methods enebled us to elongate the period of pregnancy by 3 days.When we compared the maturation of the thin ascending limb of Henle's loop between day3 neonates and day 23 fetuses, the disappearance of NKCC2 immunoreactivity in the renal medulla and diminishment of the positive transepithelial voltages in the thin ascending limb were similar.These results strongly suggest that the transformational changes of the renal medullary urine concentrating system occurring during the birth is not the process dependent on the birth itself, but the process programmed in the developmental process of the kidneys.

在我们先前的研究中，我们证明了新生儿肾髓质从肾小管功能的“禽类型”转变为“哺乳动物型”组织。转换包括活跃的NaCl重吸收的消失和大型被动NaCl在Henle环的较薄上升的肢体中的重吸收，以及通过AQP-1出现稀薄的降肢中的水渗透性。肾脏髓质的系统，我们研究了怀孕期伸长的影响，从而检查了出生对系统转化变化的影响。通过两种方法实现了怀孕的延长。一种方法是怀孕期间第17天的wag纳结扎。另一种方法是给孕妇给予怀孕大鼠。两种方法都使我们在3天之前延长了怀孕的时间。较小的升肢中的电压相似。这些结果强烈表明，出生期间发生的肾脏髓质浓缩系统的变化变化不是取决于出生本身的过程，而是肾脏发育过程中编程的过程。