Identification of the pollen determinant in the gametophytic self-incompatibility system through the analysis of self-compatible mutants in Prunus

通过李属自交亲和突变体分析鉴定配子体自交不亲和系统中的花粉决定因素

基本信息

批准号：
13460014
负责人：
TAO Ryutaro
金额：
$ 8.51万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460014/
关键词：
stone fruit S-RNase gametophytic self-incompatibility sweet cherry Japanese apricot almond Japanese plum peach

项目摘要

This study identifies the pollen determinant (pollen S) of the S-RNase based gametophytic self-incompatibility system utilizing the mutated S haplotypes. Based on the results obtained we have developed the working model for the molecular basis of self-(in)compatibility in Prunus. The summary of the results obtained is as follows.(1)Analysis of the mutated S haplotype in almondComparisons of the S locus region of a mutated Sc-hapotype with the original one identifies S haplotype-specific F-box progein gene (SFB). SFB shows the S haplotype-specific sequence polymorphism and pollen-specific expression, indicating that SFB is a very good candidate for the pollen Sin Prunus.(2)Analysis of self-compatible S haplotypes of cherry and Japanese apricotSf of Japanese apricot and S4' of sweet cherry are known to be the pollen part mutant S haplotypes. Analysis of their S locus regions revealed insertion mutations in SFBs of these S haplotypes. The insertions lead to frame shift to produce truncated transcripts for SFB, which could be the cause of self-compatibility in Sf and S4' haplotypes.(3)Self-(in)compatibility in tetraploid sour cherryIn Solanaceae, which shows the same type of gametophytic self-incompatibility as Prunus, polyploidization generally leads to self-compatibility through the so-called phenomenon, "the competitive interaction". We have analyzed self-(in)compatibility in the progeny of several crosses between several sour cherry cultivars. It appeared that the segregation of self-(in)compatibility is determined by the number of functional and non-functional S haplotypes present in the progeny, suggesting that "the competitive interaction" is absent in Prunus.(4)Working model for the molecular basis of self-(in)compatibility in PrunusBased on the above-described results, we have developed and published the working model for the genetic and molecular basis of self-(in)compatibility in Prunus.

这项研究利用突变的S单倍型确定了基于S-RNase的配子植物自我不相容性系统的花粉决定因素（花粉s）。根据获得的结果，我们为李子中自我兼容的分子基础开发了工作模型。（1）对突变的SC-型型的杏仁区域中突变的S型型在突变的SC-型型中的突变S单倍型的分析，原始一个识别S单倍型特异性F-Box Progein基因（SFB）。 SFB显示了S单倍型特异性序列多态性和花粉特异性表达，表明SFB是花粉sin runus的一个很好的候选者。（2）分析樱桃的自兼容S单倍型，日本杏子的日本杏子和日本杏子的单倍型，而甜樱桃的S4'是众所周知的，是Pollen Parten Partert part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Part Partplet saplplplept shaplplplept。对其S基因座区域的分析揭示了SFB中这些S单倍型的插入突变。 The insertions lead to frame shift to produce truncated transcripts for SFB, which could be the cause of self-compatibility in Sf and S4' haplotypes.(3)Self-(in)compatibility in tetraploid sour cherryIn Solanaceae, which shows the same type of gametophytic self-incompatibility as Prunus, polyploidization generally leads to self-compatibility through the so-called phenomenon, "the竞争互动”。我们已经分析了几个酸樱桃品种之间几个十字架的后代中的自我兼容性。似乎自我（IN）兼容性的隔离取决于后代中存在的功能和非功能s单倍型的数量，这表明在李子中不存在“竞争相互作用”。（4）基于上述结果的基于prunusby的分子基础的分子基础的工作模型，我们已经开发和出版了我们已经开发的，并且我们已经开发了我们已经开发的模型，并且我们已经开发了我们的工作模型。李子中的自我兼容性。