Study of the adhesion prevention with using self-setting barrier membrane

自凝阻隔膜防粘连的研究

• 批准号: 12470310
• 负责人: INOUE Hajime
• 金额: $ 9.09万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470310/
• 关键词: alginate membrane; adhesion; self-setting barrier membrane; osteocyte; surgical treatment; laminectomy; phagocytosis; 骨細胞

Alginate membrane is easily prepared with sodium alginate and potassium chloride. This material is easy to handle and is used in several fields. The present study focus on the preventing role of this self-setting barrier membrane in adhesion formation.In vivo study, the effect of alginate membrane during wound healing was investigated in Wistar rats. Rat paravertebral muscle was exposed on its back to establish an adhesion model. Alginate membrane was applied to the cavity over the muscle abrasion. Histological assessment showed that alginate membrane occupied the cavity fully and inflammation cells infiltrated in the first week after operation. After 3 week, giant cells came up to the edge of this membrane. Alginate membrane was almost absorbed by the phagocytosis induced by giant cells within 9 week. Compared to the control group, the granulation tissue, fibrosis and the organization of remodeling tissue were reduced. Furthermore, fat tissue replacement was well recognized in alginate membrane treated group. In another study, the prevention of neural adhesion was also investigated in Beagle dogs. Alginate membrane was applied after laminectomy. Similar histological findings were observed in epidural space and around neural tissue, to reduce adhesion with using this membrane. Taken together these data, this bioresorbable membrane has the effect to diminish the adhesion after operation.We also examined the effect of the alginate in vitro study. In the study with the osteocyte, the significant difference was not found in cell proliferation. The function of osteocyte (the production of osteocalcin and ALP) was not damaged by this membrane.Alginate membrane is forein material and the further examinations are needed for the control of inflammation and absorption. However alginate membrane is though to be one of the suitable materials for the prevention of adhesion after surgical treatments.

藻酸盐膜很容易用藻酸钠和氯化钾制备。该材料易于处理，并用于多个字段。本研究的重点是这种自我设定的屏障膜在粘附形成中的预防作用。在体内研究中，在Wistar大鼠中研究了藻酸盐在伤口愈合过程中的作用。大鼠副肌肉在其背部暴露以建立粘附模型。在肌肉磨损上将藻酸盐膜应用于腔。组织学评估表明，藻酸盐膜完全占据了空腔，并且在手术后的第一周渗透了炎症细胞。 3周后，巨细胞升到该膜的边缘。藻酸酯膜几乎被9周内巨细胞诱导的吞噬作用吸收。与对照组相比，肉芽组织，纤维化和重塑组织的组织减少。此外，在经藻酸盐膜处理的组中，脂肪组织置换良好。在另一项研究中，还研究了比格犬中的预防神经粘附。椎板切除术后施用藻酸盐膜。在硬膜外空间和神经组织周围观察到类似的组织学发现，以减少使用该膜的粘附。综合这些数据，该生物可吸收性膜具有减少操作后的粘附作用。我们还检查了藻酸盐在体外研究的作用。在与骨细胞的研究中，在细胞增殖中未发现显着差异。骨细胞（骨钙素和ALP的产生）的功能没有受到该膜的破坏。Alginate膜是前物质，控制炎症和吸收需要进一步检查。但是，藻酸盐膜是在手术治疗后预防粘附的合适材料之一。