MOLECULAR AND PATHOLOGICAL ANALYSIS OF SPINAL CORD EOSINOPHILIC LESIONS IN ATOPIC MYELITIS

特应性脊髓炎脊髓嗜酸性病变的分子和病​​理学分析

• 批准号: 12470142
• 负责人: KIRA Jun-ichi
• 金额: $ 10.24万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470142/
• 关键词: atopic myelitis; atopic dermatitis; airway allergy; eosinophils; eosinophil cationic protein; CD8 T cells; optico-spinal form of multiple sclerosis; DPB1^*0501; 運動ニューロン; Th2; eotaxin; RANTES; EDN; ECP

1) Clinicolaboratory findings of atopic myelitis with regard to the coexistence of atopic disordersWe conducted a nation-wide survey of atopic myelitis in Japan and then collected 85 cases of this myelitis. Comparison of clinicolaboratory findings between atopic myelitis with and without atopic dermatitis revealed the characteristics of this myelitis as follows ; myelitic patients with atopic dermatitis showed (1)female predominance, (2)younger age of onset, (3)preferential involvement of the cervical cord and (4)less frequent occurrences of sphincter disturbance as comopared to those without atopic dermatitis.2) Immunopathological findings of eosinophilic myelitisFive biopsid cases of eosinophilic myelitis with atopy was collected from all over the country. Immunohistological staining of all biopsied specimens revealed the perivascular lymphocyte cuffings mainly consisted of T cells. In three cases further examined for T cell subsets, CD8 T cells were predominant in the perivascular a … More reas. Moreover, large amounts of eosinophilic cationic protein was found to be deposited in the spinal cord tissues in all cases examined.3) Class II MHC genotypes in atopic myelitisThe associations with DPB1*0501 and DRB1*1501 have been demonstrated in optico-spinal and conventional form of multiple sclerosis, respectively. However, we could not demonstrate either DPB1*0501 or DRB1*1501 association with topic myelitis.4) Treatment of atopic myelitisAlthough a treatment with corticosterods was shown to shorten the duration of acute events due to multiple sclerosis, this treatment was not effective in about two thirds of patients with atopic myelitis. About eighty percentages of patients with this myelitis were successfully treated with plasma exchange. Intravenous immunoglobulin therapy combined with plasma exchange was also useful.The results of this study revealed the presence of atopy-related myeltis in Japanese, in which eosinophils were considered to play a critical role for tissue destruction like other atopic disorders, yet the precise mechanism including the spinal cord inflammation remains to be elucidated. Less

1）特应性脊髓炎的临床代理发现，关于特应性疾病的共存，对日本的特应性骨髓炎进行了全国性调查，然后收集了85例该脊髓炎。与特应特应性皮炎的特应性脊髓炎之间的临床实验室发现的比较表明，该髓炎的特征如下。表现出（1）女性占优势，（2）年轻年龄，（3）宫颈索的脊髓炎患者的优先参与颈椎的优先参与和（4）跨括约肌灾难的经常发生，因为括约肌灾难与没有特应性皮炎的患者相互症。这个国家。所有活检标本的免疫组织学染色显示，血管周淋巴细胞袖口主要由T细胞组成。在三种情况下，进一步检查了T细胞亚群，CD8 T细胞在血管周A…更多的放松中占主导地位。此外，在所有检查的所有情况下，都发现大量嗜酸性阳离子阳离子蛋白沉积在脊髓组织中。3）分别以多种层库中的光学和常规形式证明了与DPB1*0501和DRB1*1501的II类MHC基因型与DPB1*0501和DRB1*1501的关联。但是，我们无法证明DPB1*0501或DRB1*1501与主题骨髓炎相关。4）治疗特应骨髓炎的治疗，尽管证明与皮质类固醇的治疗可缩短由于多发性硬化症而导致的急性事件持续时间，但这种治疗方法无效，在大约三分之二的患者患有四分之二的患者。该脊髓炎的患者中约有80％的患者通过血浆交换成功治疗。静脉内免疫球蛋白疗法与血浆交换结合在一起也很有用。这项研究的结果表明，日语中存在与特应性相关的梅尔蒂斯的存在，其中认为嗜酸性粒细胞被认为对其他特征性疾病（如其他特征性疾病）具有至关重要的作用，但是包括脊髓索赔机制在内的精确机制仍然是尚有脊髓炎症的作用。较少的

项目成果

Horiuchi I, Yamasaki K, Osoegawa M, Ohyagi Y, Okayama A, Kurokawa T, Yamada T, Kira J: "Acute myelitis after asthma attacks with onset after puberty"J Neurol Neurosurg Psychiatry. 68. 665-668 (2000)

Horiuchi I、Yamasaki K、Osoekawa M、Ohyagi Y、Okayama A、Kurokawa T、Yamada T、Kira J：“青春期后发作的哮喘发作后的急性脊髓炎”J Neurol Neurosurg Psychiatry。

呉暁牧 他: "炎症性中枢神経疾患におけるIL-5、IL-13の検討"神経免疫学. 9. 54-55 (2001)

吴晓木等：“炎症性中枢神经系统疾病中IL-5和IL-13的检查”《神经免疫学》9. 54-55 (2001)。

吉良潤一: "別刷・医学のあゆみ 21世紀の神経免疫学"医試薬出版株式会社. 207 (2001)

吉良纯一：《重印：医学史：21世纪的神经免疫学》谏药出版有限公司207（2001）

Kira J, et al.: "Juvenile musclar atrophy of distal upper extremity (Hirayama disease) associated with atopy"J. Neuro. Neurosurg. Psychiatry. 70. 798-801 (2001)

Kira J 等人：“与特应性相关的青少年上肢远端肌肉萎缩（平山病）”J.

Wu X-M, et al: "Flow cytometric differentiation of Asian and Western types of multiple sclerosis (MS), HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and hyperIgEaemic myelitis by analysis of memory CD4 positive T cell subsets and NK

Wu X-M 等人：“通过分析记忆 CD4 阳性 T 细胞亚群和分析，流式细胞术区分亚洲和西方类型的多发性硬化症 (MS)、HTLV-1 相关脊髓病/热带痉挛性截瘫 (HAM/TSP) 和高 IgE 性脊髓炎