A study to solve the mystery of AIDS pandemic by expeditions into the most remote areas in the Congo basin.

一项通过深入刚果盆地最偏远地区的探险来解开艾滋病大流行之谜的研究。

• 批准号: 22256001
• 负责人: IDO Eiji
• 金额: $ 29.2万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22256001/
• 关键词: ウイルス; HIV; 感染症; エイズ; 進化; アフリカ; コンゴ盆地; コンゴ民主共和国; 分子進化; コンゴ民主; コンゴ; 分子疫学; コンゴ共和国; 起源

The purpose of this study is to clarify the mystery of AIDS pandemic (when, where and how did it occur?) as well as the origin of HIV. To do so, we have expedited into far remote provinces in both Democratic Republic of Congo and Republic of Congo. In addition to the conventional molecular epidemiological techniquues, we also employed the so-called second generation high-throughput DNA sequencer. So far the areas showing the most diversified profiles of HIV genotypes are the central region of Congo basin and nearby areas the majority of which is covered with the giant Congo River and its numerous branch rivers. In those areas, not only a great variety of genotypes of HIV strains are co-circulating but also new types of viruses seem to be continuously generated with high probability by way of dual infection of two different genotypic HIV strains and recombination of their genomes as a natural consequent event.

这项研究的目的是阐明艾滋病大流行的奥秘（何时，何时何地发生？）以及艾滋病毒的起源。为此，我们已经加快了刚果民主共和国和刚果共和国的遥远省份。除了传统的分子流行病学技术外，我们还采用了所谓的第二代高通量DNA序列。到目前为止，显示HIV基因型最多样化的区域是刚果盆地和附近地区的中央地区，其中大多数被巨大的刚果河及其众多分支河流覆盖。在这些领域，不仅艾滋病毒菌株的多种基因型正在共同循环，而且新型病毒似乎是通过两种不同的基因型HIV菌株的双重感染而持续产生的。自然的事件。

项目成果

Molecular Evolution of HIV-1 CRF01_AE Env in Thai Patients

• DOI: 10.1371/journal.pone.0027098
• 发表时间: 2011-11-02
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Boonchawalit, Samatchaya;Jullaksorn, Duangrat;Kameoka, Masanori
• 通讯作者: Kameoka, Masanori

A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads.

一种基于阴离子聚合物包被磁珠捕获广泛亚型和循环重组形式的 HIV-1 的技术。

• DOI: 10.3892/ijmm.2012.1009
• 发表时间: 2012
• 期刊: Int. J. Mol. Med.
• 作者: 新堀淳樹;辻 裕司;菊池 崇;荒木 徹;池田昭大;魚住禎司;R. E. S. Otadoy;歌田久司;B. M. Shevtsov;Baishev Dmitry;長妻 努;湯元清文;Sakudo A
• 通讯作者: Sakudo A

Establishment of in-house quantitative rea-time RT PCR assay for monitoring for HIV-1 viral loads and efficacy evaluation of ART in Ghanaian AIDS patients in an urban setting

建立内部定量实时 RT PCR 检测方法，用于监测城市环境中加纳艾滋病患者的 HIV-1 病毒载量和 ART 疗效评估

• 发表时间: 2011
• 作者: Barnor J;Brandful J;Ampofo W;Yamamoto N;Bonney K;Bonney M;Alale M;Ntim A;AmoahY;fori S;Addo A;Nyarko A;Ido E;Ishikawa K;Yamaoka S
• 通讯作者: Yamaoka S

Establishment of in-housequantative rea-time RT PCR assay for monitoring for HIV-1 viral loads and efficacyevaluation of ART in Ghanaian AIDS patients in an urban setting

建立内部定量实时 RT PCR 检测方法，用于监测城市环境中加纳艾滋病患者的 HIV-1 病毒载量和 ART 疗效评估

• 发表时间: 2011
• 作者: Barnor J;Brandful J;Ampofo W;Yamamoto N;Bonney K;Bonney M;Alale M;Ntim A;Amoah Y;fori S;Addo A;Nyarko A;Ido E;Ishikawa K;Yamaoka S
• 通讯作者: Yamaoka S

次世代シークエンサーを用いた臨床検体(血清)のメタゲノミック診断

使用下一代测序仪对临床标本（血清）进行宏基因组诊断

• 发表时间: 2010
• 作者: Kaihatsu K^*;Mori S;Matsumura H;Daidoji T;Kawakami C;Kurata H;Nakaya T;Kato N.;中屋隆明;中屋隆明;中屋隆明;中屋隆明
• 通讯作者: 中屋隆明