Cell Lineage Analysis of the Forebrain Structures

前脑结构的细胞谱系分析

基本信息

批准号：
14380344
负责人：
SHIMAMURA Kenji
金额：
$ 9.6万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380344/
关键词：
neuroepithelium neural stem cell cell lineage gene expression cell migration regionalization brain nucleus thalamus

项目摘要

The aim of this research project was to elucidate the origin of neurons that constitute certain brain structures upon the sheet of neuroepithelium. As a first step to this end, we have focused on the thalamic region of the brain which consists of clusters of neurons called brain nuclei to address how these neurons are specified and how these nuclear structures are formed during development. We have found that several transcription factors are expressed in the specific brain nuclei in the thalamus. The onset of their expression was found in distinct populations of the postmitotic cells in the early mantle layer of the thalamic rudiment, which was regulated by the differential activities of Sonic hedgehog in a manner consistent with its action as a morphogen. Such distinct inductive activities are mediated selectively by different Gli factors. Next we found that some neuronal populations migrate from their birthplace to the particular nuclei through highly stereotyped pathways. Transplantation experiments revealed that this migratory property is unique to the particular populations, and dependent on the surrounding environment. These results suggested a scenario that (1) the relatively simple pattern created early in development in part by inductive mechanisms leads to generation of distinct neuronal populations at defined locations within the thalamic rudiment, and (2) these distinct populations then undergo specific migration intrinsic to them, which may eventually generate more complicated patterns such as the nucleus organization in the developed thalamus. We think that these processes are likely to be basic strategies for the formation of the nuclear structures.

该研究项目的目的是阐明构成神经上皮纸上某些大脑结构的神经元的起源。作为这一目标的第一步，我们专注于大脑的丘脑区域，该区域由称为脑核的神经元组组成，以解决这些神经元的指定方式以及在发育过程中如何形成这些核结构。我们发现，在丘脑的特定脑核中表达了几个转录因子。它们的表达的开始是在丘脑序的早期地幔层的不同种群中发现的，这是由声音刺猬的差异活性以与其作为形态学作用一致的方式调节的。这种不同的诱导活性由不同的GLI因子选择性介导。接下来，我们发现一些神经元种群通过高度定型的途径从其出生地迁移到特定核。移植实验表明，这种迁移特性是特定人群所独有的，并取决于周围环境。这些结果表明了一种情况：（1）（1）在归纳机制的一部分中创建的相对简单的模式会导致在丘脑基础内定义的位置产生不同的神经元种群，以及（2）这些独特的人群然后对它们进行了特定的迁移，这可能会产生更为复杂的模式，例如nucleus the nucleus thalamus thalamus in thalamus in thalamus in thalamus。我们认为这些过程可能是形成核结构的基本策略。