Post-natal neurogenesis in the turtle spinal cord

乌龟脊髓产后神经发生

基本信息

批准号：
7596993
负责人：
RAUL E RUSSO
金额：
$ 7.08万
依托单位：
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-02-01 至 2012-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7596993
关键词：
Adolescent Adult Affect Amphibia Animals Axon Behavior Biological Models Biology Birds Birth Brain Bromodeoxyuridine Cell Line Cell Lineage Cell Proliferation Cells Central cord canal structure Complement Coupled Development Dorsal Electrophysiology (science)Embryonic Development Fiber Fishes Gap Junctions Generations Goals Green Fluorescent Proteins Growth Factor Horns Image In Vitro Injury Interneurons Knowledge Label Lesion Mammals Mediating Modeling Molecular Molecular Profiling Morphology Motor Natural regeneration Nature Neural tube Neuroglia Neuronal Differentiation Neurons Newborn Infant Oils Pattern Play Preparation Process Production Proliferating Property Radial Recovery of Function Research Role Sensory Signal Transduction Site Spinal Spinal Cord Spinal Cord Degenerative Diseases Spinal Cord Diseases Spinal Cord transection injury Staging Stem cells Structure Techniques Testing Time Tracer Turtles Vertebrates Whole-Cell Recordings cell type immunocytochemistry in vivo injured interdisciplinary approach migration molecular marker molecular phenotype neuroepithelium neurogenesis newborn neuron patch clamp progenitor protein expression receptor relating to nervous system repaired response to injury spinal cord repair stem time use voltage gated channel

项目摘要

DESCRIPTION (provided by applicant): The main hypothesis of this project proposes that the region surrounding the central canal in the spinal cord of young turtles is an active neurogenic zone in which Stem/Progenitor (S/P) cells proliferate, giving rise to immature neurons and glial cells. Connected with this hypothesis is the idea that immature neurons differentiate, migrate and integrate into pre-existing spinal circuits. Injuries and degenerative diseases of the spinal cord are among the most devastating ailments affecting many people. Understanding the biology of S/P cells in species that have retained neurogenesis after birth is crucial in order to develop adequate cell- replacement treatments. The specific aims of this project are: 1) Identification and characterization of S/P cells in the spinal cord of the turtle, 2) to determine the morphological, fine structural, molecular and functional changes taking place during differentiation of newborn neurons from S/P cells, 3) to study the migration of newborn neurons from the central canal to the parenchyma and their subsequent functional integration into spinal circuitry, and 4) to assess the effects of injury on the production and differentiation of S/P cells. This proposal takes advantage of a unique biological model and applies a multidisciplinary approach involving immunocytochemistry, fine structure analysis, time-lapse imaging and electrophysiology. Both in vivo and in vitro preparations will be used. The immature cells characterized at the electrophysiolgical level will be further analyzed with molecular-structural techniques. Putative migratory behavior of immature cells will be investigated in vitro by means of time-lapse imaging of tagged cells. Finally, the modulatory influence of injury on proliferation and differentiation of S/P cells in animals at variable time points after a complete transection of the spinal cord, will be characterized by immunocytochemical, morphological and electrophysiological techniques. The proposed research will provide important information about the generation, maturation and integration of new neurons in mature sensory-motor circuitry. This knowledge is essential to understand basic neurogenic mechanisms that may remain "dormant" in the mammalian spinal cord but could hopefully be "awakened" to alleviate spinal cord diseases.

描述（由申请人提供）：该项目的主要假设提出，年轻海龟脊髓中心管的区域是一个活跃的神经源区，在该区域中，茎/祖细胞（S/P）细胞增殖，从而引起未成熟的神经元和胶质细胞。与该假设有关的是，未成熟神经元分化，迁移并整合到先前存在的脊柱回路中。脊髓的伤害和退化性疾病是影响许多人的最具破坏性的疾病之一。了解出生后保留神经发生的物种中S/P细胞的生物学至关重要，以发展足够的细胞替代治疗。该项目的具体目的是：1）乌龟脊髓中S/P细胞的识别和表征，2）确定在分化新生儿神经元与S/P细胞分化期间发生的形态学，细胞结构，分子和功能变化，以研究新生神经元从中央流行的繁殖及其范围的繁殖效果的迁移，并研究其范围的迁移，并将其及其范围的效果迁移到近代的范围内，并构成了彼此的效果，并将其用于范围内的繁殖及其范围的效果及其范围的影响。 S/P细胞的生产和分化。该建议利用了独特的生物学模型，并采用了涉及免疫细胞化学，精细结构分析，延时成像和电生理学的多学科方法。体内和体外制剂都将使用。分子结构技术将进一步分析在电论水平上表征的未成熟细胞。未成熟细胞的推定迁移行为将通过标记细胞的延时成像在体外研究。最后，损伤对脊髓完全横断后的动物在可变时间点增殖和分化的调节作用将以免疫细胞化学，形态学和电生理技术为特征。拟议的研究将提供有关成熟感觉运动电路中新神经元的产生，成熟和整合的重要信息。这些知识对于理解哺乳动物脊髓中可能保持“休眠状态”的基本神经源机制至关重要，但希望可以“觉醒”以减轻脊髓疾病。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Modulation of gene expression during early stages of reconnection of the turtle spinal cord.

乌龟脊髓重新连接早期阶段基因表达的调节。

DOI：
10.1111/j.1471-4159.2012.07750.x
发表时间：
2012
期刊：
Journal of neurochemistry
影响因子：
4.7
作者：
García,Gabriela;Libisch,Gabriela;Trujillo-Cenóz,Omar;Robello,Carlos;Russo,RaúlE
通讯作者：
Russo,RaúlE

Spatial domains of progenitor-like cells and functional complexity of a stem cell niche in the neonatal rat spinal cord.

DOI：
10.1002/stem.1175
发表时间：
2012-09
期刊：
STEM CELLS
影响因子：
5.2
作者：
Marichal, Nicolas;Garcia, Gabriela;Radmilovich, Milka;Trujillo-Cenoz, Omar;Russo, Raul E.
通讯作者：
Russo, Raul E.

Cytological organization of the central gelatinosa in the turtle spinal cord.

龟脊髓中央胶质细胞的细胞学组织。

DOI：
10.1002/cne.21306
发表时间：
2007
期刊：
The Journal of comparative neurology
影响因子：
0
作者：
Trujillo-Cenoz,Omar;Fernandez,Anabel;Radmilovich,Milka;Reali,Cecilia;Russo,RaulE
通讯作者：
Russo,RaulE

Neural reconnection in the transected spinal cord of the freshwater turtle Trachemys dorbignyi.