Studies on origin and coevolution of Schistosoma japonicum in Asia

亚洲日本血吸虫起源与协同进化研究

• 批准号: 13576008
• 负责人: AGATSUMA Takeshi
• 金额: $ 7.1万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13576008/
• 关键词: Schistosomatidae; Schistosoma; Orientobilharzia; avian schistosome; mammalian schistosome; molecular phylogenetic tree; origin of Schistosoma; Coevolution; アジア; 日本住血吸虫; 起源; 進化; ミトコンドリアDNA; リボソームRNA遺伝子; 住血吸虫類; 28S; ITS2; CO1; 塩基配列; COI

To date, in order to examine the origin of the genus Schistosoma, phylogenetic relationships among the genus Schistosoma have been estimated using partial nucleotide sequences of CO1, 1srDNA and ITS2. In this study, whole nucleotide sequences of CO1, 1srDNA aqnd ssrDNA were determined for the first time, and on the basis of the sequence information, phylogenetic relationships were inferred among species from the family Schistosomatidae. Materials used in this study are in total 29 species in 10 genera consisting of 6 genera from the subfamily Schistosomatinae, two genera from the subfamily Bilharziellinae and two genera from the subfamily Gigantobilharziinae. The genus Chimaerohemecus in the family Sanguinicolidae was used as an outgroup. The number of nucleotides determined for CO1, ssrDNR and 1srDNA was 1122 bp and 3765 bp, respectively. In this study, combined nuleotide sequences including all of the three genes were used for tree analysis. It was estimated that the mammalianschistosomes were paraphyletic, since the clad of (Ornithobilharzia+Austrobilharzia) was supported to be a sister clad of (Schistosoma+Orientobilharzia). It was also found that Orientobilharzia was placed within the genus Schistosoma, and a sister of clad consisting of the African group and the S.indicum group. This result suggests that Orientobilharzia could be an ancestral species for them.There are two theories on the origin of Schistosoma. One is the African origin theory by Davis (1980,1992), proposing that Indian continental plate, which separated from the Gondwana Land, carried an ancestral species of Schistosoma from Africa to Asia. Another is the Asian origin theory (Snyder & Loker,2000; Agatsma et al., 2003; Locker et al., 2004) proposing that an ancestral species of Schistosoma occurred in Asia and expanded Asia to Africa through migration of a mammalian host. The present study supported the Asian origin theory again.

迄今为止，为了检查血吸虫属的起源，已经使用CO1，1SRDNA和ITS2的部分核苷酸序列估算了血吸虫属之间的系统发育关系。在这项研究中，首次确定了CO1的全核苷酸序列，1srDNA AQND ssrDNA，并根据序列信息，从Schistosomatidae家族中推断出系统发育关系。这项研究中使用的材料共有10种属中的29种，由6个属组成，来自亚家族斑点体，来自Bilharziellinae亚家族的两个属和两个属的属，来自gigantobilharziinae亚家族。 Sanguinicolidae家族中的Chimaerohemecus属被用作外群。确定CO1，SSRDNR和1SRDNA的核苷酸的数量分别为1122 bp和3765 bp。在这项研究中，包括所有三个基因（包括所有三个基因）的合并的Nuleotide序列用于树分析。据估计，自从（Ornithobilharzia+Opterobilharzia）的外壳被支持是（Schistosoma+Orientobilharzia）的姐妹。还发现Orientobilharzia被放置在Scistosoma属内，并且是由非洲群体和S.Indicum群体组成的clad姐妹。这一结果表明，东方ob骨可能是它们的祖先物种。有关血吸虫起源的两种理论。一个是戴维斯（1980,1992）的《非洲起源理论》，他提出与冈瓦纳土地分开的印度大陆板将祖先的血吸虫物种从非洲到亚洲。另一个是亚洲起源理论（Snyder＆Loker，2000; Agatsma等，2003; Locker等，2004）提出，亚洲祖传物种发生在亚洲，并通过哺乳动物宿主的迁移将亚洲扩展到非洲。本研究再次支持亚洲原籍理论。

项目成果

Phylogenetic status of a lung fluke in the philippines based on mitochondrial genome.

基于线粒体基因组的菲律宾肺吸虫的系统发育状况。

• 发表时间: 2003
• 期刊: Japanese Journal of Tropical Medicine and Hygiene 31
• 作者: Yukita Sato;Moritoshi Iwagami;Jun Iwashita;Tatsuya Abe;Masayoshi Yukawa;Bayani L.Blas;Kenjiro Kawashima;Takeshi Agatsuma
• 通讯作者: Takeshi Agatsuma

Takeshi Agatsuma: "Origin and evolution of Schistosoma japonicum (Review)"Parasitology International. 52. 335-340 (2003)

Takeshi Agatsuma：“日本血吸虫的起源和进化（评论）”国际寄生虫学。

Mitochondrial gene content,arrangement and composition compared in Africa and Asian schistosomes.

非洲和亚洲血吸虫线粒体基因含量、排列和组成比较。

• 发表时间: 2001
• 期刊: Molecular&Biochemical Parasitology 117
• 作者: T.H.Le;P-F.Humair;D.Blair;T.Agatsuma;D.T.J.Littlewood;D.P.McManus
• 通讯作者: D.P.McManus

A melecular phylogeographic study based on DNA sequences from individual metacercariae of Paragonimus mexicanus from Guatemala and Ecuador.

基于危地马拉和厄瓜多尔墨西哥并殖吸虫个体囊蚴 DNA 序列的分子谱系地理学研究。

• 发表时间: 2003
• 期刊: Journal of Helminthology, Mar. 77(1)
• 作者: M.Iwagami;C.MOnroy;M.A.Rosas;M.R.Pinto;A.G.Guevara;J.C.Vieira;Y.Agatsuma
• 通讯作者: Y.Agatsuma

Molecular taxonomic position of the elephant schistosome,Bivitellobilharzia nairi,newly discovered in Sri Lanka.

斯里兰卡新发现的大象血吸虫 Bivitellobilharzia nairi 的分子分类地位。

• 发表时间: 2004
• 期刊: Parasitology International 53
• 作者: Takeshi Agatsuma;R.P.V.J.Rajapakse;V.Y.Kuruwita;Moritoshi Iwagami;R.C.Rajapakse
• 通讯作者: R.C.Rajapakse