The characterization of female-specific gene fs 800 in Schistosoma

血吸虫女性特异性基因 fs 800 的表征

• 批准号: 03670194
• 负责人: AGATSUMA Takeshi
• 金额: $ 1.15万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670194/
• 关键词: Schistosoma mansoni; Female-specific gene; Chorion gene; fs 800 gene; fs800; 日本住血吸虫; DNA; 卵殻タンパク遺伝子; クロ-ニング

Egg production of worm pairs is a major cause of pathogenesis in schistosomiasis. Thus an understanding of female reproductive development, especially mechanisms of female-specific gene regulation is of major importance in disease control. A previously isolated cDNA that encodes the fs 800 protein was used to identify and isolate a recombinant phage containing an entire copy of the fs 800 gene. The gene is contained on a 5.5 kbp EcoRI fragment. There are no introns. The cap-site of the mRNA was determined by primer extension using RNA as template and dideoxy sequencing. The upstream regions of the gene contain putative cis-acting elements (TCACGT, GTAGAAT) that have been shown to play a role in the regulation of chorion gene expression in insects. The other elements that share a common core motif, TRRAAT(R=pyrimidine), are also found. These elements have been shown to bind nuclear proteins and control quantitative and qualitative changes in reporter gene expression in silkmoths.

成对产卵是血吸虫病发病的主要原因。因此，了解女性生殖发育，特别是女性特异性基因调控机制对于疾病控制至关重要。使用先前分离的编码 fs 800 蛋白的 cDNA 来鉴定和分离含有完整 fs 800 基因拷贝的重组噬菌体。该基因包含在 5.5 kbp EcoRI 片段中。没有内含子。 mRNA 的帽位点通过使用 RNA 作为模板的引物延伸和双脱氧测序来确定。该基因的上游区域包含推定的顺式作用元件（TCACGT、GTAGAAT），这些元件已被证明在昆虫绒毛膜基因表达的调节中发挥作用。还发现了具有共同核心基序的其他元件 TRRAAT(R=嘧啶)。这些元件已被证明可以结合核蛋白并控制蚕蛾报告基因表达的定量和定性变化。

项目成果

吾妻 健,P.T.Loverde: "マンソン住血吸虫卵形成に関与する雌性特異的遺伝子fs800のcharacterization" 寄生虫学雑誌. 40. 90 (1991)

Ken Azuma，P.T.Loverde：“曼氏血吸虫卵子发生中涉及的女性特异性基因 fs800 的特征”《寄生虫学杂志》40. 90 (1991)。

T.Agatsuma & P.T.Loverde: "The characterization of a Schistosoma mansom' femaleーspecific gene,fs800" Experimental Parasitology. (1992)

T.Agatsuma 和 P.T.Loverde：“血吸虫女性特异性基因的特征，fs800”实验寄生虫学（1992）。

T.Agatsuma: "Characterization of a Schistosoma mansoni female-specific gene (fs 800) involved in egg shell protein production." Jpn.J. Parasitol. 40. 90 (1991)

T.Agatsuma：“曼氏血吸虫女性特异性基因 (fs 800) 参与蛋壳蛋白生产的表征。”

T.Agatsuma & K.Terasaki: "Clonal diversity of triploids of the Japanese Fasciola species and relationships with other species of Fasciola" International Journal for Parasitology. (1992)

吾妻

吾妻 健: "マンソン住血吸虫卵形成に関与する雌性特異的遺伝子fs800のcharacterization" 寄生虫学雑誌. 40. 90 (1991)

Ken Azuma：“曼氏血吸虫卵子发生中涉及的女性特异性基因 fs800 的表征”《寄生虫学杂志》40. 90 (1991)。