The role of c-Met oncogene expression in the secondary palate shelves adhesion process.

c-Met癌基因表达在次级腭架粘附过程中的作用。

基本信息

批准号：
11470397
负责人：
YOSHIDA Hideo
金额：
$ 6.66万
依托单位：
Tokushima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

BALB/C mouse was used in the identification of the localization of HGF, c-Met, etc. in the mouse foetus secondary palate shelves. The foetus is exposed from the dam from pregnancy 14th to 15th in 6 hour by anesthetize, and the paraffin section is produced, and HGF, c-Met oncogene protein, the cytokeratin which is the epithelial cell marker, vimentin which is the mesenchymal cell marker were examined by immunostaining procedure. As the result, there was the expression in which c-Met was mainly extensive for whole palate shelves before the adhesion 14th for HGF in the epitherial layer of palate shelves, and the vimentin observed the mesenchymal tissue, and the cytokeratin was recognized only in the epitherial layer. The adhesion took the horizontal positioning on secondary palate shelves near, and c-Met expression was strengthened. The expression of HGF is abated a little right after the adhesion in the epitherial layer, and it increase mesenchyme layer a little. The expression of c-Met … More reversely decreased in the mesenchymal organization a little, and it was strengthened in the epitherial layer. There was some the resistant expression on the vimentin with the adhesion division epithelium in the circumference. And, there was the coloration on the cytokeratin in the epitherial layer. HGF, c-Met was abated in the adhesion completion in the whole. Vimentin there palate whole area expression, so that the cytokeratin may diffuse from the adhesion division, there mesenchymal extensiv, that is to say, epithelium marker cytokeratin mesenchymal simultaneously, there identical expression, it was indicated that HGF, c-Met induced the conversion of epithelium-mesenchymal tissue. It was indicated that the apoptosis was concerned in that it extensively appears palate shelves 14th and that the protrusion moves from the vertical position to horizontal positioning on Nick-End labelling which is an index to the apoptosis. In addition, HGF gene expression was confirmed in DNA-RNA in situ hybridization. Less

BALB/C小鼠用于鉴定小鼠胎儿次级腭架中HGF、c-Met等的定位。胎儿从怀孕14日至15日经麻醉、石蜡从母体中暴露。制作切片，检查 HGF、c-Met 癌基因蛋白、上皮细胞标记物细胞角蛋白、间充质细胞标记物波形蛋白免疫染色结果显示，在腭架上皮层中，HGF在第14位粘附之前，c-Met主要在整个腭架中广泛表达，波形蛋白在间质组织中观察到，细胞角蛋白也有表达。仅在上皮层中发现粘附，靠近次级腭架的水平定位，并且c-Met表达增强，HGF的表达略有减弱。上皮层粘附后，间质层c-Met表达略有增加，间质组织中c-Met表达略有下降，上皮层有一定抗性表达。周围有粘连分裂上皮的波形蛋白，上皮层细胞角蛋白着色，c-Met 减弱。 Vimentin在整个腭区都有表达，这样细胞角蛋白就可以从粘连分裂处扩散出来，间质出现广泛分布，也就是说，上皮细胞角蛋白间质同时出现相同的表达，这表明HGF、c -Met诱导上皮-间质组织的转化，这表明细胞凋亡主要与第14号腭架有关。 Nick-End标记上的突起从垂直位置移动到水平位置，这是细胞凋亡的指标。另外，在DNA-RNA原位杂交中证实了HGF基因表达。