Study on the associated regulation of energy and metabolism of glucose and lipids

能量与糖脂代谢相关调控研究

基本信息

批准号：
10470235
负责人：
INOUE Gen
金额：
$ 8.32万
依托单位：
Research Institute for Production Development
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1.Using leptin-overexpressing transgenic mice and KKA^γ mice, we examined the effects of leptin on the development of obesity and diabetes. Leptin overexpressing KKA^γ mice became obese as KKA-γ mice did, while the development of diabetes in leptin overexpressing KKA^γ mice was significantly delayed compared to KKA^γ mice. The result suggests that leptin may be useful for the prevention of diabetes.2.We found that insulin stimulated IRS1-associated PI3 kinase activity was increased in liver and skeletal muscles of leptin transgenic mice and themice intra cranially injectedwithieptin. However, could not find changes in Akt activity or the expression of constituents of intracellular insulin signal in liver and skeletal muscles of leptin transgenic mice. The results imply the divergent effects of leptin on insulin signaling system.3.We already developed transgenic mice expressing UCP3 in skeletal muscles, and are going to explore the roles of energy expenditure on the regulation of glucose and lipid metabolism.Ninna-ji Temple was founded in 888 AD. by the Emperor Uda at the request of his father, the former Emperor Kk, in the northwestern suburbs of the Capital-City Heian. Ninna-ji Temple hierarchically occupied the highest position among the, nonzeki temples whose abbots were members of the Impenal Family until the end of the Edo era, therefore the research of the documents which have been preserved at Ninna-ji Temple for centuries (the Ninna-ji Documents in general) contributes not only to the historical study of the temple, but also to the study of Japanese history.

1。使用过表达的转基因小鼠和KKA^γ小鼠，我们检查了瘦素对肥胖和糖尿病发育的影响。过表达KKA^γ小鼠的瘦素像KKA-γ小鼠一样拜拜，而与KKA^γ小鼠相比，瘦素过表达KKA^γ小鼠的糖尿病的发育显着延迟。结果表明，瘦素可能对预防糖尿病有用。2。我们发现，胰岛素刺激的IRS1相关PI3激酶活性在瘦素转基因小鼠的肝脏和骨骼肌中增加了，并且它们会在颅内注射。但是，找不到Akt活性的变化或细胞内胰岛素信号传导和瘦素转基因小鼠的骨骼肌的成分的表达。结果意味着瘦素对胰岛素信号系统的不同作用。3。我们已经开发了在骨骼肌中表达UCP3的转基因小鼠，并将探索能量消耗在调节葡萄糖和脂质代谢的调节中的作用。尼纳 - JI寺庙在888 AD中建立。由UDA皇帝应他父亲的要求，前KK皇帝在西北海安（Heian）西北郊区。在江户时代结束之前，忍者在非西基寺庙中占据了无国内家族的成员的最高位置，因此，在尼娜·吉神庙（Ninna-Ji Temple）保存了几个世纪的文档的研究（一般而言）对尼尔纳·吉（Ninna-ji）的历史研究不仅贡献了对这座庙宇的历史研究，但也对寺庙的历史贡献了。