Studies on the development of functional biopolymers and the removal of pathogenic viruses in the water environment

功能性生物聚合物开发及去除水环境中病原病毒的研究

• 批准号: 10450194
• 负责人: OMURA Tatsuo
• 金额: $ 8.45万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10450194/
• 关键词: Virus-binding proteins; Poliovirus 1; Capsid proteins; Affinity chromatography; SDS-PAGE; ELISA method; Activated sludge; Interaction between proteins; バイオポリマー; ウイルス; 吸着; 細胞外タンパク質; アフィニティークロマトグラフィー; 機能性; タンパク質; アミノ酸配列; FPLC

Most of pathogenic viruses in the sewage sludge is removed through the activated sludge process. This removal of pathogenic viruses is mainly due to the adsorption to the sludge flocs. It is said that this adsorption is based on the interaction between capsid proteins of viruses and proteins included in the sludge flocs. There is high possibility that the proteins in the sewage sludge can be utilized for the removal of pathogenic viruses from the water environment.The objectives of this research were to survey the virus-binding protein (VBP) from the activeted sludge and to resolve the binding mechanism between the VBP and pathogenic viruses.Following conclusions were obtained in this research :-VBPs were isolated from the activated sludge culture by the affinity chromatography technique.-VBPs were classified into two groups by the molecular weight (estimated by SDS-PAGE). They had the molecular weights around 110,000 Da and over 170,000 Da, respectively.-It became obvious by ELISA method that VBPs could bind poliovirus 1 two times as efficinently as the crude proteins extracted from activated sludge culture.-Isolation points of VBPs were pH 7.0 and 7.7. This result indicated that the binding of pathogenic viruses by VBPs was based on the interaction between proteins such as hydrophobic bond and hydrogen bond.

污水污泥中的大部分病原病毒通过活性污泥工艺被去除。这种病原病毒的去除主要是由于污泥絮体的吸附作用。据说这种吸附是基于病毒的衣壳蛋白与污泥絮凝物中所含的蛋白质之间的相互作用。污水污泥中的蛋白质很有可能用于去除水环境中的致病病毒。本研究的目的是从活性污泥中调查病毒结合蛋白（VBP）并解析其结合蛋白。本研究得到以下结论： -通过亲和层析技术从活性污泥培养物中分离出VBP。 -根据分子量（通过SDS-PAGE估计）将VBP分为两组。它们的分子量分别约为 110,000 Da 和超过 170,000 Da。 -通过 ELISA 方法明显看出，VBP 与脊髓灰质炎病毒 1 的结合效率是从活性污泥培养物中提取的粗蛋白的两倍。 - VBP 的分离点为 pH 7.0和7.7。这一结果表明，VBPs与病原病毒的结合是基于蛋白质之间的相互作用，如疏水键和氢键。

项目成果

安附太郎,佐野大輔,福士謙介,大村達夫: "銅吸着能を有する活性汚泥中タンパク質の検索"環境工学研究論文集. 第37巻. 131-140 (2000)

Taro Yasutsuki、Daisuke Sano、Kensuke Fukushi、Tatsuo Omura：“寻找具有铜吸附能力的活性污泥中的蛋白质”《环境工程研究杂志》，第 37 卷，131-140（2000 年）

D.Sano, K.Fukushi, K.Yano, Y.Yoshida and T.Omura: "Enhanced Virus Recovery from Municipal Sewage Sludge with a Combination of Enzyme and Cation Exchange Resin"Water Science and Technology. 43-2. 75-82 (2001)

D.Sano、K.Fukushi、K.Yano、Y.Yoshida 和 T.Omura：“结合酶和阳离子交换树脂增强城市污水污泥中的病毒回收”水科学与技术。

安附太郎,熊谷幸博,大村達夫: "銅添加培養された活性汚泥微生物が産出するタンパクの銅吸着能力評価"土木学会第55回年次学術講演会講演概要集. (2000)

Taro Antsuki、Yukihiro Kumagai、Tatsuo Omura：“添加铜培养的活性污泥微生物产生的蛋白质的铜吸附能力的评估”日本土木工程师学会第 55 届年度学术会议摘要（2000 年）。

佐野大輔,福士謙介,大村達夫: "Enzymatic Virus Elution (EVE法) による下水汚泥からの腸管系ウイルス誘出"土木学会年次講演会講演集. 第7巻. 120-121 (1999)

Daisuke Sano、Kensuke Fukushi、Tatsuo Omura：“通过酶病毒洗脱（EVE 方法）从污水污泥中提取肠道病毒”日本土木工程师学会年会论文集，第 7 卷，120-121（1999 年）。

S.Kato, K.Fukushi and T.Omura: "Isolation of Virus-Binding Proteins from Activated Sludge Culture"Proceedings of 1st World Water Congress of the International Water Association. Vol.7. 30-31 (2000)

S.Kato、K.Fukushi 和 T.Omura：“从活性污泥培养物中分离病毒结合蛋白”国际水协会第一届世界水大会论文集。