Approach for bone replacing apaite cement

阿帕特骨水泥替代骨的方法

• 批准号: 09470438
• 负责人: ISHIKAWA Kunio
• 金额: $ 7.81万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09470438/
• 关键词: Apaite cement; Bone replacement; Osteoblasts; Osteoclasts; Carbonate apatite; Differentiation; アパタイト; 増殖; 炭酸水素ナトリウム; 機械的性質; 結晶性; 硬化時間

The aim of this research is to find out the feasibility of the development of apatite cement (AC) that could replace with bone in relatively short period. First, osteoclasts were incubated on the surface of set conventional AC (c-AC), fast-setting AC (fs-AC) anti-washout AC (aw-AC). Sintered apatite and cortical bone were also used as reference materials. We found osteoclasts resorped AC even though osteoclasts formed no resorption pits on the surface of sintered apaite. However, the area of resorption was much smaller when compared with that of cortical bone. No significant difference was found based on the type of AC indicating that all AC would be replaced with bone in the same rate. To accelerate the resorption of AC with osteoclasts, we then introduced carbonate ion into AC.The resorption area increased with contents of carbonate upto 6%. However the mechanical strength of AC decreased with increase in the amount of added carbonate. With respect to the bone formation that should occur after resoption of AC with osteoclasts, we evaluated differentiation of osteoblasts on the surface of AC.We found AC enhance the differentiation of osteoblasts. We therefore concluded that there is a good chance to fabricate apatite cement that could be replaced with bon in relatively short periods.

这项研究的目的是找出可以在相对较短的时间内用骨头代替的磷灰石水泥（AC）的可行性。首先，将破骨细胞在集合常规AC（C-AC）的表面孵育，快速设定的AC（FS-AC）抗冲洗AC（AW-AC）。烧结的磷灰石和皮质骨也被用作参考材料。即使在烧结的apaite表面没有吸收坑，我们发现破骨细胞吸收了AC。但是，与皮质骨相比，吸收面积要小得多。根据AC的类型，未发现所有AC将以相同速率代替骨骼的显着差异。为了加速破骨细胞的AC吸收，我们将碳酸盐离子引入AC中。吸收面积随碳酸盐含量高达6％而增加。但是，随着添加碳酸盐量的增加，AC的机械强度降低了。关于用破骨细胞重塑后应发生的骨形成，我们评估了AC表面上成骨细胞的分化。我们发现AC增强了成骨细胞的分化。因此，我们得出的结论是，有很大的机会在相对较短的时间内可以用BON代替磷灰石水泥。