The development of therapy for inflammatory bal tissue with Paneth cell-protection factorsowel disease using cultured epitheli

培养上皮细胞潘氏细胞保护因子治疗炎症性巴尔组织疾病的进展

• 批准号: 23890012
• 负责人: TANABE Hiroki
• 金额: $ 2.08万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23890012/
• 关键词: 移植・再生医療; プロテオーム; 生理活性; 再生医療; 細胞・組織

The aim of this research is to analyze the mechanisms in regeneration of crypt structures in the gut, and to establish novel culture system with maintaining the normal crypt structure.In this study, intestinal villi and crypt are exfoliated from mouse small intestine and the crypt-rich fractions are collected for the culture. Small intestinal crypts are mixed in Marti-gel at 37 degree, and covered with stem cell selection media. The small intestinal crypts are cultured without cell isolation. Several factors in culture media are checked and R-spondin is found to be a critical factor for culture system.To establish the animal model, the tissues of dog and pig are analyzed pathologically. Paneth cells with rich granules are observed in the porcine intestine but in the dog. Pig is one of the candidates for animal model alternative to human research. The development of animal model is highly expected in future.

本研究的目的是分析肠道隐窝结构再生的机制，并建立维持正常隐窝结构的新型培养系统。收集丰富的级分用于培养。小肠隐窝在 37 度的 Marti-gel 中混合，并用干细胞选择培养基覆盖。小肠隐窝无需细胞分离即可培养。对培养基中的多种因素进行了检查，发现R-spondin是培养系统的关键因素。为了建立动物模型，对狗和猪的组织进行了病理学分析。在猪肠中观察到颗粒丰富的潘氏细胞，但在狗中观察到。猪是替代人类研究的动物模型候选者之一。动物模型的发展未来备受期待。

项目成果

Trypsin: Structure, Biosynthesis Functions

胰蛋白酶：结构、生物合成功能

• 发表时间: 2012
• 作者: 柳川-松田彩;黒嶋雄志;安田元昭;北村哲也;進藤正信;東野史裕;Hiroki Tanabe
• 通讯作者: Hiroki Tanabe

Trypsin:Structure, Biosynthesis and Functions. Trypsin acts as an activating enzyme in gut innate immunity

胰蛋白酶：结构、生物合成和功能。

• 作者: Structure;Biosynthesis and Functions. Trypsin acts as an activating enzyme in gut innate immunity. Hiroki Tanabe;Mikihiro Fujiya and Yutaka Kohgo
• 通讯作者: Mikihiro Fujiya and Yutaka Kohgo

Trypsin acts as an activating enzyme in Gut Innate Immunity

胰蛋白酶在肠道先天免疫中充当激活酶

• 发表时间: 2012
• 作者: H Tanabe;M Fujiya;Y Kohgo
• 通讯作者: Y Kohgo