Functional proteomic analysis to identify memory formation-related proteins in the Drosophila mushroom bodies.

功能蛋白质组分析可识别果蝇蘑菇体中与记忆形成相关的蛋白质。

• 批准号: 22800012
• 负责人: YAMAZAKI Daisuki
• 金额: $ 2.01万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22800012/
• 关键词: 記憶学習; ショウジョウバエ; プロテオミクス; CREB; 記憶・学習

I started to develop the new methods to approach the molecular or cellular mechanisms underlying memory formation, storage, retrieval in this study. I tried to identify new factors related to learning and memory by proteomics analysis and then, I planed to determine the cells in which they would function. But I failed to establish the methods to concentrate the synapse-specific proteins. So I changed my approach into identify the cells recruited into memory traces. I generated CREB activity reporter flies and succeeded in labeling the cells in response to long-term memory formation.

我开始开发新的方法来处理本研究中记忆形成，存储，检索的分子或细胞机制。我试图通过蛋白质组学分析来确定与学习和记忆有关的新因素，然后，我计划确定它们功能的细胞。但是我未能建立浓缩突触特异性蛋白的方法。因此，我将方法更改为识别募集到内存痕迹的单元格。我产生了CREB活性报告，并成功地响应了长期记忆形成而标记细胞。

项目成果

Acute Inhibition of PKA Activity at Old Ages Ameliorates Age-Related Memory Impairment in Drosophila

• DOI: 10.1523/jneurosci.3229-10.2010
• 发表时间: 2010-11
• 期刊: The Journal of Neuroscience
• 作者: D. Yamazaki;J. Horiuchi;T. Miyashita;M. Saitoe

Long-term memory formation-dependent changes of CREB reporter signals in Drosophila

果蝇中 CREB ​​报告信号的长期记忆形成依赖性变化

• 发表时间: 2011
• 作者: Daisuke Yamazaki;Makoto Hiroi;Toshiharu Ichinose;Tetsuya Tabata
• 通讯作者: Tetsuya Tabata