Development of novel organic phases for high selective and sensitive HPLC by controlled surface modification

通过受控表面修饰开发用于高选择性和灵敏 HPLC 的新型有机相

• 批准号: 19205011
• 负责人: IHARA Hirotaka
• 金额: $ 31.2万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19205011/
• 关键词: 液体クロマトグラフィー; 分子認識; 界面化学; ナノ構造制御; コア・シェル微粒子; 分子ゲル; 超分子化学; 微粒子; 液体クロマトグラフィ; コア・シェル; 分子識別; 界面単分子膜; コア・シェル粒子; 有機・無機ハイブリッド; コアシェル構造; 懸濁重合法; シリカ; ナノコンポジット; ポリスチレン; クロマトグラフィー; 懸濁重合; ラフネス界面

This research project aims to develop a new class of HPLC packing materials with high selectivity. To accomplish this purpose, we proposed two goals :(1) Physical control of surface morphology and (2) chemical modification by graft polymerization on supporting materials and HPLC applications.1. We established a new method for creation of core-shell particles with silica particles-layer shells. This method has several advantages that can be emphasized as follows :(a) the core-shell particles can be readily prepared by one-pot suspension polymerization.(b) The resultant shell is stably immobilized by covalent bonding.(c) By proper choosing the diameter and combination of silica particles, the shell structures can be adjusted from single-to multi-layers.(d) By using porous silica particles and removal of silica particles, the dimple structural surface can be created.2. For chemical functionalization of silica surface, a polymer-grafting method has been investigated. Vinyl monomers with heterocyclic structures such as carbazole and oxazole were used. The resultant hybrids showed excellent separation ability in HPLC especially for polyaromatic hydrocarbons. It has also been established that the selectivity can be tuned by not only choosing a monomer but also polymerization method. The most successful results were observed in the base-line separation of tocopheroles containing β- and γisomers.

该研究项目旨在开发一类具有高选择性的新型高效液相色谱填充材料。为了实现这一目的，我们提出了两个目标：(1) 表面形态的物理控制；(2) 通过接枝聚合对支撑材料和高效液相色谱应用进行化学改性。 .1.我们建立了一种用二氧化硅颗粒层壳制备核壳颗粒的新方法，该方法具有以下几个优点：(a)可以通过一锅法轻松制备核壳颗粒。悬浮聚合。(b)所得产物(c)通过适当选择二氧化硅颗粒的直径和组合，可以将壳结构从单层调整为多层。(d)通过使用多孔二氧化硅颗粒并去除二氧化硅颗粒， 2.对于二氧化硅表面的化学功能化，研究了具有杂环结构的乙烯基单体如咔唑和恶唑的接枝方法。所得杂化物在 HPLC 中表现出优异的分离能力，尤其是对于聚芳烃。还确定，不仅可以通过选择单体，而且可以通过聚合方法来调节选择性，在含有生育酚的基线分离中观察到了最成功的结果。 β-和γ异构体。

项目成果

Complete chromatographic separation of steroids including 17α-and 17β-estradioles using carbazoie-based polymeric organic phase in both revered and normal phase HPLC.

在崇尚和正相 HPLC 中，使用卡巴唑基聚合物有机相对包括 17α-和 17β-雌二醇在内的类固醇进行完全色谱分离。

• 发表时间: 2010
• 期刊: Analytical and Bioanalytical Chemistry
• 影响因子: 4.3
• 作者: A.K.Mallik;K.Shingo;U.G.Gautam;T.Sawada;M.Takafuji;H.Ihara
• 通讯作者: H.Ihara

Preparation of high selective HPLC packing materials based on altemating copolymer-grafted silica

交替接枝二氧化硅高选择性HPLC填料的制备

• 发表时间: 2010
• 期刊: Journal of Separation Science
• 影响因子: 3.1
• 作者: A.K.Mallik;M.Takafuji;H.Ihara
• 通讯作者: H.Ihara

Evaluation of selectivity for 1-glutamide-derived highly ordered assemblies in reversed-phase high-performance liquid Chromatography

反相高效液相色谱中 1-谷氨酰胺衍生的高度有序组装体的选择性评估

• 发表时间: 2009
• 期刊: Talanta 77
• 作者: Doi;M.;M. Mori;篠原久典;蜂谷正樹;M.Mizanur Rahman
• 通讯作者: M.Mizanur Rahman

Glutamide Derivatives as Novel Molecular Recognitive Packing Materials for RP-HPLC

谷酰胺衍生物作为 RP-HPLC 的新型分子识别填充材料

• 发表时间: 2007
• 作者: Atsuro Takai;Claude P. Gros;Roger Guilard;and Shunichi Fukuzumi;Abul K.Mallik
• 通讯作者: Abul K.Mallik

表面にディンプルを有する粒子の製造方法及び表面にディンプルを有する粒子

表面具有凹痕的颗粒的制造方法及表面具有凹痕的颗粒

• 发表时间: 2009