Development of novel organic phases for high selective and sensitive HPLC by controlled surface modification

通过受控表面修饰开发用于高选择性和灵敏 HPLC 的新型有机相

基本信息

批准号：
19205011
负责人：
IHARA Hirotaka
金额：
$ 31.2万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2010
项目状态：
已结题

项目摘要

This research project aims to develop a new class of HPLC packing materials with high selectivity. To accomplish this purpose, we proposed two goals :(1) Physical control of surface morphology and (2) chemical modification by graft polymerization on supporting materials and HPLC applications.1. We established a new method for creation of core-shell particles with silica particles-layer shells. This method has several advantages that can be emphasized as follows :(a) the core-shell particles can be readily prepared by one-pot suspension polymerization.(b) The resultant shell is stably immobilized by covalent bonding.(c) By proper choosing the diameter and combination of silica particles, the shell structures can be adjusted from single-to multi-layers.(d) By using porous silica particles and removal of silica particles, the dimple structural surface can be created.2. For chemical functionalization of silica surface, a polymer-grafting method has been investigated. Vinyl monomers with heterocyclic structures such as carbazole and oxazole were used. The resultant hybrids showed excellent separation ability in HPLC especially for polyaromatic hydrocarbons. It has also been established that the selectivity can be tuned by not only choosing a monomer but also polymerization method. The most successful results were observed in the base-line separation of tocopheroles containing β- and γisomers.

该研究项目旨在开发具有高选择性的新型HPLC包装材料。为了实现这一目的，我们提出了两个目标：（1）对表面形态的物理控制，（2）通过在支撑材料和HPLC应用上进行移植聚合化的化学修饰1。我们建立了一种用二氧化硅颗粒壳壳创建核壳颗粒的新方法。该方法具有多种优势，可以强调如下：（a）可以通过一锅悬浮液聚合而容易制备核心壳颗粒。（b）通过合适的固定粘合。（c）通过使用硅胶的直径和组合来适当地选择孤立的孤立的孤立（silice sini bialial by silice by silie bialial biallial lidi lidi lidi）。颗粒，可以创建昏暗的结构表面2。对于二氧化硅表面的化学功能化，已经研究了一种聚合物接种方法。使用乙烯基单体具有杂环结构，例如甲状腺癌和恶唑。最终的杂种在HPLC中表现出出色的分离能力，尤其是对于多芳烃。还已经确定了选择性不仅可以通过选择单体，而且可以通过聚合方法来调节。最成功的结果是在含有β-和γ异构体的大量的基本分离中观察到的。