DEVELOPMENT OF CLINICAL SYSTEM FOR MESENCHYMAL STEM CELL REGENERATIVE THERAPY IN RESORBED ALVEOLAR BONE

再生牙槽骨间充质干细胞再生治疗临床系统的开发

• 批准号: 16209057
• 负责人: HOSOKAWA Ryuji
• 金额: $ 30.53万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16209057/
• 关键词: REGENARATIVE DENTISTRY; BONE REGENERATION; DENTISTRY; GALECTINE; CHROMOGRANIN A; ORAL HEALTH RELATED QOL; OHIP; PARAFUNCTION; 幹細胞; 再生医学; トランスレーショナルリサーチ; トランスレーションリサーチ; 再生医療; 歯科用インプラント; 間葉系細胞; PRP

We have studied on several aspects of clinical application system development for regenerative therapy in dentistry. The summary of the results is as follows ;1) We evaluate the reliability and validity of a Japanese short version of the Oral Health Impact Profile (OHIP-JP16) for regenerative therapy in dentistry. The results showed that internal consistency and reproducibility demonstrated quite a good reliability. As a result of having examined for behaviormetrics study, OHIP-JP16 showed good measurement properties and might be appropriate for use in the clinical settings.2) We investigated the effects of galectin-9 on osteoblast proliferation and its signaling mechanisms. Galectin-9-induced proliferation of the obtained osteoblasts in a dose-dependent manner, whereas galectin-1, -3, and -4 did not. Galectin-9-induced phosphorylation of c-Src and subsequent ERK1/ERK2 in the osteoblasts. Galectin-9-induced clustering of lipid rafts detected by cholera toxin B (CUB; binding the raft-resident ganglioside GM1) using confocal microscopy. These results suggest that galectin-9, but not other galectins, induced proliferation of human osteoblasts through clustering lipid rafts on membrane and subsequent phosphorylation of the c-Src/ERK signaling pathway.3) Bruxism and parafunctions are potential risk factors for implant / regenerative therapy, therefore we investigated the relationship between bruxism behavior and a salivary stress biomarker level There was a positive correlation between self-reported bruxism and self.reported jaw functional limitation. The CgA level was significantly negative in correlation with bruxism behavior. Our results suggest that daytime physiological stress level is significantly negative in correlation with sleep bruxism behavior.

我们研究了用于牙科再生治疗的临床应用系统开发的几个方面。结果的摘要如下：1）我们评估了日本简短版本的口腔健康影响概况（OHIP-JP16）的可靠性和有效性，用于牙科再生治疗。结果表明，内部一致性和可重复性表现出非常好的可靠性。由于检查了行为学研究研究，OHIP-JP16表现出良好的测量特性，并且可能适合在临床环境中使用。2）我们研究了lectectin-9对成骨细胞增殖及其信号传导机制的影响。 Galectin-9诱导的成骨细胞的增殖以剂量依赖性方式，而Galectin-1，-3和-4则没有。 CALECTIN-9诱导的C-SRC的磷酸化以及成骨细胞中的ERK1/ERK2。通过霍乱毒素B（CUB；结合筏居住的神经节GM1结合）检测到的脂蛋白-9诱导的脂质筏的聚类。这些结果表明，甘油9（而不是其他甲状腺素）通过将脂质筏聚集在膜上以及随后的C-SRC / ERK信号传导途径的磷酸化中引起人类成骨细胞的增殖。3）自我报告的磨牙和自我之间的正相关。报告的下巴功能限制。 CGA水平与磨牙行为的相关性显着阴性。我们的结果表明，白天的生理压力水平在与睡眠磨牙行为的相关性中显着阴性。

项目成果

Effects of Sleep Bruxism Behavior on Salivary Physiological Stress Biomarker Level

睡眠磨牙行为对唾液生理应激生物标志物水平的影响

• 作者: MAKINO M;et. al.
• 通讯作者: et. al.

Interaction of galectin-9 with lipid rafts induces osteoblast proliferation through the c-Src/ERK signaling pathway

• DOI: 10.1359/jbmr.071008
• 发表时间: 2008-02-01
• 期刊: JOURNAL OF BONE AND MINERAL RESEARCH
• 影响因子: 6.2
• 作者: Tanikawa, Rena;Tanikawa, Takahisa;Tanaka, Yoshiya
• 通讯作者: Tanaka, Yoshiya

ミニマルインターベンションインプラント患者中心の治療戦略ガイド

以患者为中心的微介入种植治疗策略指南

• 发表时间: 2007
• 作者: 細川 隆司;他
• 通讯作者: 他

Effects of Sleep Bruxism Behavior on Salivary Physiological Stress Biomarker

睡眠磨牙行为对唾液生理应激生物标志物的影响

• 期刊: Int.Journal of Prosthodontics (In press)
• 作者: Makino;M.;et. al.
• 通讯作者: et. al.

Reliability and Validity of the OHIP 16

OHIP 16 的可靠性和有效性

• 发表时间: 2006
• 作者: TAKENEKA M;et. al.
• 通讯作者: et. al.