General Understanding of Two-component Signal Transduction System

二元信号传导系统的一般认识

• 批准号: 16208009
• 负责人: MIZUNO Takeshi
• 金额: $ 31.37万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16208009/
• 关键词: Two-component Sianal Transduction; Phosphorelay; Stress Responses; Fungi; Arabidopsis; Plant Hormones; Plant Circadian Clock; 植物; オルガネラ分化; 情報伝達; サイトカイニン; 二成分情報伝達; 酸化ストレス; 酵母; 高等植物; シグナル伝達; 大腸菌; 時計分子機構; 嫌気応答; 開花制御; 浸透圧応答

(A): According to the well-documented scenario with regard to the cytokinin-mediated phosphorelay signal transduction in Arabidopsis thaliana, certain members of the type-B ARR family are crucially implicated in the regulatory networks that are primarily propagated by the cytokinin-receptors (AHKs) in response to cytokinin. Here we focused on a pair of highly homologous ARR10 and ARR12 genes by constructing an appropriate arr10 and arr12 double null mutant. The results suggested (A) that ARR10 and ARR12 are the prominent players that act redundantly in the AHK-dependent cytokinin signaling in roots.(B) : In Arabidopsis thaliana, the flowering time is regulated through the circadian clock that measures day-length and modulates the photoperiodic CO-FT output pathway in accordance to the external coincidence model. Nevertheless, little is known about the genetic linkage between the major clock associated TOCJ, CCAI, and LHY genes and the . canonical CO-FT flowering pathway. By employing a … More set of mutants including an extremely early flowering tocl ccal thy triple mutant, here we showed that CCAI and LHY act redundantly as negative regulators for the photoperiodic flowering pathway. The results of this study suggested that the circadian clock might open an exit for a photoperiodic output pathway during daytime.(C) : For a model filamentous fungi, Aspergillus nidulans, here we first compiled a whole list of His-Asp phosphorelay components, uncovering 15 genes for His-kinase (HK), 4 genes for response regulator (RR), and only one for histidine-containing phosphotransfer intermediate (HPt) . For all of these RR genes, a set of deletion mutants was constructed so as to create a null allele for each. When examined these mutant strains, two of them (designated as AsskA and AsrrA) showed a marked phenotype of hypersensitivity to oxidative stresses (particularly, hydrogen peroxide) .These results together are indicative of a physiological importance of the His-Asp phosphorelay signaling network involving the SskA and SrrA response regulators. Less

(A)：根据拟南芥中细胞分裂素介导的磷酸中继信号转导的详细记录，B 型 ARR 家族的某些成员与主要由细胞分裂素受体传播的调控网络密切相关(AHK) 响应细胞分裂素在这里，我们通过构建适当的 arr10 和 ARR12 基因来关注一对高度同源的 ARR10 和 ARR12 基因。 arr12 双无效突变体。结果表明 (A) ARR10 和 ARR12 是在根部 AHK 依赖性细胞分裂素信号传导中发挥冗余作用的重要参与者。(B)：在拟南芥中，开花时间是通过生物钟调节的。然而，我们对相关主时钟之间的遗传联系知之甚少。 TOCJ、CCAI 和 LHY 基因以及规范的 CO-FT 开花途径这项研究的结果表明，生物钟可能在白天打开光周期输出途径的出口。(C)：对于丝状模型。真菌，构巢曲霉，在这里我们首先编制了His-Asp磷酸传递元件的完整列表，发现了15个His激酶基因（HK），4个反应调节基因（RR），以及仅一个含组氨酸磷酸转移中间体（HPt）的基因。对于所有这些 RR 基因，构建了一组缺失突变体，以便为每个突变体创建无效等位基因。当检查这些突变体菌株时，其中两个（指定为）。 AsskA 和 AsrrA) 显示出对氧化应激（特别是过氧化氢）过敏的显着表型。这些结果共同表明了涉及 SskA 和 SrrA 反应调节因子的 His-Asp 磷酸中继信号网络的生理重要性。

项目成果

Molecular basis for promoter selectivity of the transcriptional activator OmpR of Escherichia coli.

大肠杆菌转录激活子 OmpR 启动子选择性的分子基础。

• 发表时间: 2005
• 期刊: J.Biochem.(Tokyo) 137
• 作者: Kawai;Y.他;Ohashi K.その他
• 通讯作者: Ohashi K.その他

ARKS His-kinase regulates root elongation through an ETR1-dependent integrated abscisic acid and ethylene signaling pathway in Arabidopsis thaliana

ARKS His-kinase 通过 ETR1 依赖性整合脱落酸和乙烯信号通路调节拟南芥根伸长

• 发表时间: 2007
• 期刊: Plant Cell Phvsiol 48
• 作者: Iwama;A.;Yamashino;T.;Tanaka;Y.;Sakakibara;H.;Kakimoto;T.;Sato;S.;Kato;T.;Tabata;S.;Nagatani;A.;Mizuno;T
• 通讯作者: T

Identification of amino acid substitutions that render the Arabidopsis cytokinin receptor histidine kinase AHK4 constitutively active

• DOI: 10.1093/pcp/pcm145
• 发表时间: 2007-12-01
• 期刊: PLANT AND CELL PHYSIOLOGY
• 影响因子: 4.9
• 作者: Miwa, Kumiko;Ishikawa, Kuniko;Mizuno, Takeshi
• 通讯作者: Mizuno, Takeshi

Characterization of NikA histidine kinase implicated in the phosphorelay signal transduction of Aspeergillus nidulans with special reference to fungicide responses.

与构巢曲霉磷酸中继信号转导有关的 NikA 组氨酸激酶的表征，特别涉及杀菌剂反应。

• 发表时间: 2007
• 期刊: Biosci. Biotechnol. Biochem. 71(印刷中)
• 作者: Hagiwara;D.;Mizuno T.(10/10)
• 通讯作者: Mizuno T.(10/10)

AHK5 His-kinase regulates root elongation through an ETR1-dependent integrated abscisic acid and ethylene signaling pathway in Arabidopsis thaliana.

在拟南芥中，AHK5 His-激酶通过 ETR1 依赖性整合脱落酸和乙烯信号通路调节根伸长。

• 发表时间: 2007
• 期刊: Plant Cell Physiol 48
• 作者: Iwama;et. al.
• 通讯作者: et. al.