Molecular mechanism for adsorption and adsorption of soil components formed on the surface of equipment wall during manufacturing and development of an efficient cleaning method

制造过程中设备壁表面形成的土壤成分吸附吸附的分子机理及高效清洗方法的开发

基本信息

批准号：
16206076
负责人：
NAKANISHI Kazuhiro
金额：
$ 29.95万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

项目摘要

In various manufacturing processes, cleaning of the equipment and piping systems used for manufacturing of various products is requisite for maintaining sanitary conditions and/or functions of the equipments. In particular, in the food- and bio-products manufacturing processes, a large amount of energy and detergents are usually consumed in addition to time, since soils or residues of the products strongly interact with the equipment surface. In this study, we studied adsorption mechanisms of various proteins/peptides and low-molecular weight substances as a model soil component on the surfaces of various metal oxides, plastics, and glasses. We clarified the molecular mechanisms for reversible/irreversible adsorptions, states and orientation of the molecules adsorbed, the effects of the pI values of the metal oxide surfaces/solution pHs on the adsorption characteristics, and conformational change of the adsorbed proteins, on the basis of adsorption/desorption experiments, IR spectrum analyses, and molecular calculations. Furthermore, we investigated the effects of various factors such as the kinds of the co-existing salts and their concentrations on the removal rates of the metal oxide surface fouled with proteins, using H2O2-electrolysis cleaning method that was developed by the author's group. Furthermore, we developed a method to immobilize proteins with controlled orientation and structure for various uses in chip-based biotechnology, which was derived in the course of investigation on the adsorption mechanism of proteins.

在各种制造过程中，用于维持设备的卫生条件和/或功能是必需的，用于制造各种产品的设备和管道系统。特别是，在食品和生物产品制造过程中，由于土壤或产品的残留物与设备表面强烈相互作用，因此通常会消耗大量能量和洗涤剂。在这项研究中，我们研究了各种蛋白质/肽和低分子重量物质的吸附机制，作为各种金属氧化物，塑料和玻璃表面上的模型土壤成分。我们阐明了分子的分子机制，可逆/不可逆的吸附，状态和吸附的分子取向，金属氧化物表面/溶液pHS的PI值对吸附特性的影响，以及基于吸附蛋白的构象变化，基于吸附剂/质量分析量和分析量的分析。此外，我们研究了作者组开发的H2O2-2-OCTROLSOSIS清洁方法，研究了各种因素的影响，例如共存盐的种类及其浓度对用蛋白质构成的金属氧化物表面的去除速率。此外，我们开发了一种方法，将基于芯片的生物技术的各种用途固定蛋白质，该蛋白质是在研究蛋白质吸附机理的过程中得出的。