An analysis of the cells derived from the olfactory placode and its possibl correlation of the migrating LHRH neurons.

对源自嗅觉基板的细胞的分析及其与迁移的 LHRH 神经元的可能相关性。

基本信息

批准号：
06680793
负责人：
MURAKAMI Shizuko
金额：
$ 0.9万
依托单位：
Juntento University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

The actual migration of LHRH neurons from the nasal epithelium to the forebrain was demonstrated in the chick embryo using fluorescent dye-labeling. There were a number of labeled cells which did not co-express LHRH.These results suggest that some non-LHRH neurons in the forebrain are of olfactory placodal origin. In order to identify the other cell types migrating into the forebrain together with LHRH neurons, the tissue distribution of somatostatin (SST) immunoreactivity was studied in the nasal and forebain region in the chick embryo. On embryonic day (ED) 3.5, SST immunoreactive (-ir) cells and -ir fibers appeared in the olfactory epithelium and olfactory nerve bundles. At ED6-8, one component of the SST-ir fibers was found to separate from the olfactory nerve and it entered the medial forebrain surface. During this same period, a few SST-ir cells were observed in the medial forebrain adjacent to the SST-ir fibers. SST immunoreactivity in the nasal and forebrain areas was most striking at ED5-8 but it virtually disappeared by the day of hatching. These results indicate that the expression of SST in the nasal and forebrain region is transient in the chick embryo. Since the SST-ir cells did not co-express LHRH, it appears that SST-ir cells belong to a different cell population from LHRH neurons. However, a close relationship exist between SST-ir neural elements and LHRH neurons. This may play a role in development of LHRH neurons. Additionally, the appearance of the SST-ir cells in the forebrain at ED6-8 suggest their migraiton from the nasal region.

使用荧光染料标记，在雏鸡的胚胎中证明了LHRH神经元从鼻上皮到前脑的实际迁移。有许多没有共表达LHRH的标记细胞。这些结果表明，前脑中的某些非LHRH神经元是嗅觉斑点的起源。为了鉴定其他细胞类型与LHRH神经元一起迁移到前脑，研究了生长抑素（SST）免疫反应性的组织分布在雏鸡胚胎中的鼻和前巴区域。在胚胎日（ED）3.5，SST免疫反应（-iR）细胞和-IR纤维出现在嗅觉上皮和嗅觉神经束中。在ED6-8处，发现SST-IR纤维的一个组成部分与嗅觉神经分开，并进入内侧前脑表面。在同一时期，在与SST-IR纤维相邻的内侧前脑中观察到了一些SST-IR细胞。鼻腔和前脑区域中的SST免疫反应性最为震撼，在ED5-8处最引人注目，但在孵化的那天实际上消失了。这些结果表明，鼻腔中SST的表达在雏鸡胚胎中是短暂的。由于SST-IR细胞未共表达LHRH，因此SST-IR细胞似乎属于与LHRH神经元不同的细胞群体。但是，SST-IR神经元素与LHRH神经元之间存在密切的关系。这可能在LHRH神经元的发展中发挥作用。此外，在ED6-8处的前脑中SST-IR细胞的出现表明它们来自鼻部区域。