Physiological and Biochemical Assessment of Membrane Barrier Function in Inflammatory Disease

炎症性疾病中膜屏障功能的生理生化评估

• 批准号: 06672280
• 负责人: HAYASHI Masahiro
• 金额: $ 1.34万
• 依托单位: Faculty of Pharmaceuticla Sciences, Science University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672280/
• 关键词: Inflammatory Bowel Disease Model; Membrane Resistance; Short Circuit Current; Damage Score; Electrophysiology; Membrane Permeability; Paracellular Pathway; 粘膜炎症; 免疫抑制酸性蛋白; 膜電位

Membrane barrier function and transport ability for electrolytes were assessed in inflammatory bowel disease. For the inflammatory rat models prepared with trinirobenzene sulfonic acid or acetic acid, relation between the electro-physiological parameter, membrane potential difference, short circuit current (Isc), or membrane resistance (Rm), and the degree of morphological inflammation (damage score) was compared. The parameters decreased with increase in the scores, but the correlation was not significant, resulting that the electro-physiological analysis was considered more objective and accurate. For the acetic acid-models, the decreased Rm recovered to the control value in 4 days after the preparation and the increased permeability of FITC-dextran (FD-4) recovered to the control in 7 days. Accordingly, it was shown that recovery from the disease in the relatively short term can be assessed by the above method. The increase in the permeability of FD-4 and the decrease in Rm suggested the enlargement of the paracellular pathway. The membrane damage by diclofenac sodium (DC) was assessed by the above method. Release of cellular protein was found after perfusion of DC in the rat. However, Isc was increased by the presence of theophylline, suggesting that the membrane metabolizing ability remained normal. In conclusion, the application of the above method is useful in the quantitative assessment of various inflammatory disease.

在炎症性肠病中评估了电解质的膜屏障功能和运输能力。对于用三罗苯磺酸或乙酸制备的炎症大鼠模型，比较了电 - 物理学参数，膜电位差，短路电流（ISC）或膜耐药性（RM）与形态炎症（损害评分）的程度之间的关系。参数随分数的增加而降低，但是相关性并不显着，导致电生理分析被认为更客观和准确。对于乙酸模型，在制备后的4天内恢复了RM的降低，并且在7天内恢复了FITC-DEXTRAN（FD-4）的渗透性增加。因此，结果表明，可以通过上述方法评估相对短期内从疾病中恢复。 FD-4的渗透性增加和RM的降低表明细胞细胞途径的扩大。双氯芬酸钠（DC）的膜损伤通过上述方法评估。 DC在大鼠灌注后发现细胞蛋白的释放。但是，茶碱的存在增加了ISC，表明膜代谢能力保持正常。总之，上述方法的应用可用于对各种炎症性疾病的定量评估。

项目成果

Mikio Tomita,et al.: "Absorption-Enhancing Mechanism of Sodium Caprate and Decanyolcarnitine in Caco-2 Cells" J.Pharmacol.Exp.Therap.272. 739-743 (1995)

Mikio Tomita 等人：“Caco-2 细胞中癸酸钠和癸醇肉碱的吸收增强机制”J.Pharmacol.Exp.Therap.272。

Hiroshi Kumagai et al.: "Assessment of Mucosal Function in Some Experimental Colitis Models" Digestion & Absorption. 17 (2). 113-115 (1994)

Hiroshi Kumagai 等人：“某些实验性结肠炎模型中粘膜功能的评估”消化

熊谷 博,他: "各種大腸炎モデルにおける粘膜機能評価" 消化と吸収. 17. 113-115 (1994)

Hiroshi Kumagai 等人：“各种结肠炎模型中粘膜功能的评估”《消化和吸收》17. 113-115 (1994)。

Mikio Tomita et al.: "Absorption-Enhancing Mechanism of Sodium Caprate and Decanyl-carnitine in Caco-2 Cells" J.Pharmacol. Exp. Therap.272 (2). 739-743 (1995)

Mikio Tomita 等人：“Caco-2 细胞中癸酸钠和癸基肉碱的吸收增强机制”J.Pharmacol。

Mikio Tomita et al.: "Absorption-Enhancing Mechanism of Sodium Caprate and Decanylcarnitine in Caco-2 Cells" J. Pharmacol. Exp. Therap.272. 739-743 (1995)

Mikio Tomita 等人：“Caco-2 细胞中癸酸钠和癸酰肉碱的吸收增强机制”J. Pharmacol。