Urogenital Tumors Studies by Growth Factors and Their Receptors Contents in the Tumos

泌尿生殖肿瘤生长因子及其受体含量的研究

基本信息

批准号：
06671611
负责人：
YOSHIDA Ken-ichiro
金额：
$ 1.47万
依托单位：
Dokkyo University (1995)Saitama Medical University (1994)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671611/
关键词：
Renal cell carcinoma Epidermal growth factor Transforming growth factor-alpha Epidermal growth factor receptor Prognosis α型腫瘍増殖因子(TGF-α)予後因子 EGF TGF-α EGF受容体前立腺肥大症オートクリン

项目摘要

A Scatchard analysis using membrane fractions of human renal cell tissues and isotope labeled EGF revealed that EGFR was detected in 38 of 52 human renal cell carcinoma (RCC), while EGFR was not detected in most of the normal renal tissues. The kd value in the order of 10^<-10>M and the maximum binding sites with the order of fmol/mg membrane protein were observed. When compared EGFR levels with pathohistological findings, no significant correlations were observed between them, except between Fuhrman's nuclear grades and EGFR levels and that EGFR levels in pM1 was significantly higher than pM0. So far 13 patients died of the disease, all were EGFR detected cases. These results suggest the difficulty in predicting potients'prognosis by the pathohistological findings, but the biochemical evaluation of the tumor migth predict the patients'outcome. No significant differences in EGFR levels were observed between the primary and metastatic sites of RCC, while the administration of interferon-alpha (IFN) decreased EGFR levels in the metastatic sites, suggesting that the antitumor effects of IFN on RCC might be mediated through EGFR.The immunohistochemical stainings of EGF and TGF-alpha for normal, primary and metastatic sites of renal tissues demonstrated that TGF-alpha in the normal tissues was not stained but EGF was weakly stained at tubular cells and glomerulus, while both of them were stained in the RCC.The staining grades, especially for TGF-alpha, were much higher in the metastatic sites than the primary sites, suggesting that an autocrine mechanism must be involved for the tumor growth in the metastatic sites.

使用人肾细胞组织的膜部分和同位素标记的 EGF 进行的 Scatchard 分析显示，在 52 个人肾细胞癌 (RCC) 中的 38 个中检测到 EGFR，而在大多数正常肾组织中未检测到 EGFR。观察到kd值约为10^-10M，最大结合位点约为fmol/mg膜蛋白。将EGFR水平与病理组织学结果进行比较时，除了Fuhrman核分级和EGFR水平之间以及pM1中EGFR水平显着高于pM0之外，没有观察到它们之间存在显着相关性。迄今已有13名患者死于该病，均为EGFR检测病例。这些结果表明通过病理组织学结果预测患者的预后存在困难，但肿瘤的生化评估可能预测患者的结果。 RCC原发部位和转移部位的EGFR水平没有观察到显着差异，而干扰素-α（IFN）的施用降低了转移部位的EGFR水平，表明IFN对RCC的抗肿瘤作用可能是通过EGFR介导的。肾组织正常部位、原发部位和转移部位的 EGF 和 TGF-α 免疫组化染色结果表明，正常组织中 TGF-α 未染色，而 EGF 在肾小管细胞和肾小管细胞处呈弱染色。肾小球，而肾细胞癌中两者均被染色。转移部位的染色等级，尤其是 TGF-α，远高于原发部位，表明转移部位肿瘤的生长必须涉及自分泌机制。