Senescence of osteocyte and dyamism of nuclear proteins

骨细胞的衰老和核蛋白的动力

• 批准号: 06671437
• 负责人: CHIGIRA Masaki
• 金额: $ 1.34万
• 依托单位: Gunma University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671437/
• 关键词: Senescence; Nuclear Proteins; Protein-free Culture; Apoptosis; Cell Cycle; General theory; 骨芽細胞; 細胞死; アポトーシス; 信号伝達; 分化

In order to induce cellular senescence, complete protein-free cuture of sarcoma clone was performed. Electron-microscopic and flow-cytometric analyzes revealed that the reduction in growth was accomanied by the appearance of apoptotic cells. However, no internucleosomal fragmentation was observed. Pulse field gel electrophoresis revealed that cleavage of DNA into high-molecular-weight fragments estimated as 50 to 150 kilobase was found in the protein-free cells. Flow cytometry using Hoechst 33342 showed a direct correlation between the large DNA fragmentation and apoptosis. Inhibitor analysis suggest that the apoptosis involves a specific endogenous endonuclease.On the other hand, theoretical analysis of bone remodeling using morphometry revealed that so-called osteoporosis is due to an adaptational process of bone. Under limited bone mass, expansion of external and internal diameters optimize bone mechanical strength calculated by area and moment inertia.

为了诱导细胞衰老，进行了肉瘤克隆的完全无蛋白质切割。电子 - 微观和流动性分析表明，凋亡细胞的出现使生长的减少降低。但是，未观察到核小体碎裂。脉冲场凝胶电泳表明，在无蛋白质细胞中发现了DNA裂解为估计为50至150千个酶的高分子重量片段。使用HOECHST 33342的流式细胞仪显示出大的DNA片段化和凋亡之间的直接相关性。抑制剂分析表明，凋亡涉及特定的内源性核酸酶。另一方面，使用形态计量学对骨头重塑的理论分析表明，所谓的骨质疏松症是由于骨的适应性过程。在有限的骨骼质量下，外部和内径的膨胀优化了按面积和力矩惯性计算的骨骼机械强度。

项目成果

Chigira M: "Mechanical optimization of bone." Med.Hypoth.(in press.).

Chigira M：“骨骼的机械优化。”

Watanabe H, Kanbe K, et al.: "Apoptosis of a fibrosarcoma induced by protein-free culture involves DNA cleavage to large" Int. J. Cancer,. 62. 191-198 (1995)

Watanabe H、Kanbe K 等人：“无蛋白培养物诱导的纤维肉瘤细胞凋亡涉及 DNA 裂解为大片段”Int。

Chigira M: "Mechanical optimization of bone." Med. Hypoth.(in press). (1996)

Chigira M：“骨骼的机械优化。”

M.CHIGIRA: "DIFFERENTIATION AS' SYM BIOSIS." MEDICAL HYPOTHESIS'. 43. 17-18 (1994)

M.CHIGIRA：“分化为‘SYM BIOSIS’。”

Watanabe H,Kanbe K,Shinozaki T,Hoshino H,Chigira M: "Apoptosis of a fibrosarcoma induced by protein-free culture involves DNA cleavage to large fragments but not internucleosomal fragmentation." Int.J.Cancer. 62. 191-198 (1995)

Watanabe H、Kanbe K、Shinozaki T、Hoshino H、Chigira M：“无蛋白培养物诱导的纤维肉瘤细胞凋亡涉及 DNA 裂解成大片段，但不涉及核小体间片段化。”