A histochemical study on the expression and localization of growth factors

生长因子表达和定位的组织化学研究

基本信息

批准号：
06670014
负责人：
ISEKI Shoichi
金额：
$ 1.34万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

We have used both immunohistochemistry and in situ hybridization techniques to determine the sites of localization of immunoreactivity and mRNA signal of various growth factors in the entire rat body, and have obtained the following results :The expression of hepatocyte growth factor (HGF) was demonstrated in the submandibular gland of rats. Both the mRNA signal and immunoreactivity for HGF were localized to the epithelial cells of granular convoluted tubules. This finding added HGF to the list of many growth factors produced in the rodent submandibular gland.As an attempt for the electron microscopic in situ hybridization, the cryostat sections of rat submandibular gland were first treated for in situ hybridization with ^<35>S-labelled EGF probe and then embedded in epoxy resin for electron microscopic radioautography. The result suggested that this method is more useful for identifying morphologically the type of mRNA-positive cells than determining the subcellular localization of mRNA.An immunohistochemical study using an antibody against acidic fibroblast growth factor (aFGF) revealed that subpopulations of endocrine cells in the pancreas and small intestinal mucosa are positively immunostained in their secretory granules. This result suggested that an aFGF-like substance is produced by the cell populations that express glucagon-precursor.The expression of vascular endothelial growth factor (VEGF) is very low in the normal rat submandibular gland. However, a stimulation of submandibular gland to grow by isoproterenol caused a transient rise in the rate of expression of VEGF in this organ that peaked at 1 h after the stimulation. The mRNA was localized to the acinar cells. This result suggested a certain relation between isoproterenol-induced growth of submandibular gland and VEGF effects on the blood vessels.

我们已经使用了免疫组织化学和原位杂交技术来确定整个大鼠体内各种生长因子的免疫反应性和mRNA信号的位置，并获得了以下结果：在大鼠的下颌骨中证明了肝细胞生长因子（HGF）的表达。 HGF的mRNA信号和免疫反应性均位于颗粒状曲折小管的上皮细胞上。这一发现将HGF添加到了啮齿动物下颌腺体中产生的许多生长因子列表中。作为试图进行电子显微镜原位杂交，首先对大鼠下颌腺的低温恒温器切片进行了与 ^<35> s-s-s-s-labell egf探针的原位杂交进行电子杂交，然后在Elepoxy in eproxy中进行了启动。结果表明，这种方法对于在形态上识别mRNA阳性细胞的类型比确定mRNA的亚细胞定位更有用。一种免疫组织化学研究使用针对酸性成纤维细胞生长因子（AFGF）的抗体研究表明，潘氏氏菌和小型内分泌细胞的亚饮食表明，pancrema和小型内分泌细胞的分泌属于氏菌，他们的分泌性均在饲养性上。该结果表明，一种表达胰甘蓝素晶状体的细胞群体产生了类似AFGF的物质。在正常大鼠下颌腺体中，血管内皮生长因子（VEGF）的表达非常低。然而，刺激下颌腺可以通过异丙肾上腺素生长，导致该器官中VEGF表达速率的短暂上升，该刺激在刺激后1小时达到峰值。 mRNA位于腺泡细胞中。该结果表明，异丙肾上腺素诱导的下颌腺生长与VEGF对血管的影响之间存在一定的关系。