Developing a genetic toolbox for Aeromonas salmonicida subsp. pectinolytica to analyze function and interplay of its native SMC-complexes Smc-ScpAB and MukBEF

开发杀鲑气单胞菌亚种的遗传工具箱。

• 批准号: 442665864
• 负责人: Dr. Franziska Kemter
• 金额: --
• 依托单位: Department of Fundamental Microbiology
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/442665864?language=en
• 关键词: Developing; genetic; toolbox; Aeromonas; salmonicida

SMC protein complexes condense chromosomes by binding and translocating along the DNA and thereby are an essential part of compaction of the DNA of a cell. The both most important SMC-complexes in bacteria, Smc-ScpAB and MukBEF, compact the DNA in a different manner: While Smc-ScpAB aligns both chromosome arms, MukBEF condenses each chromosome arm separately. In contrast to all other bacteria, which contain either Smc-ScpAB or MukBEF, species of Aeromonas harbor both SMC-complexes. This co-appearance allows the analysis of differences in function and roles of Smc-ScpAB and MukBEF regarding chromosomal segregation and conformation, as well as their coordination in the same system. To allow such an analysis in Aeromonas, genetic tools like plasmids, inducible promoters and techniques for genetic manipulation of Aeromonas are needed. Hence, the aim of this project is to establish a genetic toolbox for Aeromonas salmonicida subsp. pectinolytica and to analyze the function and interplay of Smc-ScpAB and MukBEF.

SMC 蛋白复合物通过沿着 DNA 结合和易位来压缩染色体，因此是细胞 DNA 压缩的重要组成部分。细菌中最重要的 SMC 复合物 Smc-ScpAB 和 MukBEF 以不同的方式压缩 DNA：Smc-ScpAB 对齐两个染色体臂，而 MukBEF 分别压缩每个染色体臂。与含有 Smc-ScpAB 或 MukBEF 的所有其他细菌相反，气单胞菌属物种同时含有 SMC 复合物。这种共同出现允许分析 Smc-ScpAB 和 MukBEF 在染色体分离和构象方面的功能和作用的差异，以及它们在同一系统中的协调。为了在气单胞菌中进行此类分析，需要质粒、诱导型启动子等遗传工具和气单胞菌遗传操作技术。因此，该项目的目的是建立杀鲑气单胞菌亚种的遗传工具箱。 pectinolytica 并分析 Smc-ScpAB 和 MukBEF 的功能和相互作用。