DEVELOPMENT OF AN ELECTROCHEMICAL MEASUREMENT METHODS ABOUT BIOFUNCTIONAL MATERIALS AND THEIR APPLICATION TO NONAQUEOUS SOLUTION SYSTEMS

生物功能材料电化学测量方法的开发及其在非水溶液体系中的应用

基本信息

批准号：
06640778
负责人：
NAKAMURA Toshio
金额：
$ 1.28万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Studies for getting basic data concerning to redox and electron transfer about biofunctional materials and for developing sensors for use both in detection of those and in tracing their activities were carried out. Biofunctional materials, which are generally difficuit to exchange electrons between those and an electrode, were contained in fairly a thin film of hydrophilic polymer, polyacrylamide (PAA)coated on a platinum disk electrode. A cell was constructed with the electrode and an acentonitrile(AN) solution to investigate electrochemical functions. 1)A PAA and methylviologen were dissolved with water on a platinum disk and dried in a sil-ica gel descicator. From the cyclic voltammetry (CV) the cell constructed with the electrode was confirmed to share a necessary condition for carring out the research. 2)The platinum disk electrodes coated with a PAA membrane containing a heme protein (catalase, bleomycin, hemoglobin, or cytochrome c) were constructed. It was found from the electrode that CV behavior about the redox of oxygen were different each other according to a containing protain. It should be nescessary to investigate the effect against the fedox behavior of oxygen by the difference of protains and the charge of the iron. 3)The platinum disk electrodes coated with a PAA membrane con-taining some functional materials(NADH,NAD,PQQ,diaphrase)were constructed. It was found from the electrode that redox peak currents were proportional to the con-centration of the cyclohexanol in AN.4)The PAA in gel type used in the experiment as a matrix for fixing biofunctional materials close to platinum disk was investi-gated to elucidate electrochemical basic characteristics.(Pacifichem'95, part 1, ANYL 193(1995). (Honolulu, Dec 17-22))Informations about redox behavior of a cho-lesterol and an oxygen monoxide were also obtained by the platinum electrode coated with a PAA containing cobalt phthalocyanine or hemoglobine.

进行有关生物功能材料的氧化还原和电子转移的基本数据的研究，以及开发传感器用于检测这些材料和追踪其活动的传感器。生物功能材料通常很难在电极之间交换电子，并包含在镀膜电极上的亲水性聚合物，聚丙烯酰胺（PAA）的薄膜中。用电极和乙腈（AN）溶液构建细胞，以研究电化学功能。 1）将PAA和甲基化学剂用水溶解在铂盘上，并在Sil-ica凝胶后代干燥。从循环伏安法（CV）中，确认用电极构造的细胞有一个必要的条件来掌握研究。 2）构建了含有含有血红素蛋白的PAA膜（过氧化氢酶，博霉素，血红蛋白或细胞色素C）的铂盘电极。从电极发现，根据含有蛋白质的氧化物氧化还原的CV行为彼此不同。通过蛋白质的差异和铁的电荷来研究对氧气的氧气行为的影响。 3）构建了含有PAA膜的某些功能材料（NADH，NAD，PQQ，Diaphrase）的铂盘电极。从电极中发现，氧化还原峰值与在AN.4）中的环己醇的对集合成比例。 17-22））有关CHO-性酚和一氧化碳的氧化还原行为的信息，还通过含有含有PAA的PAA的铂电极获得了含有钴苯甲酸钴的PAA或血红蛋白的信息。