THE ROLE OF TRANSCRIPTIONAL REGULATION FACTORS ON ONTOGENY,DIFFERENTIATION AND ONCOGENESIS ; ITS APPLICATION FOR HISTOPATHOLOGIC DIAGNOSIS

转录调控因子对个体发育、分化和癌发生的作用；

• 批准号: 05807014
• 负责人: HAYASHI Yoshitake
• 金额: $ 1.09万
• 依托单位: KOBE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05807014/
• 关键词: HNF-1; vHNF-1; Competitive RT-PCR; Transcriptional regulation factor; Hepatocellular carcinoma; Hepatoblastoma; Histopathology; Human Pathology; Competetive RT-PCR; 病理; 転写制御

The hepatic transcription of human albumin and alpha-fetoprotein (AFP) genes is controlled by a complex set of regulatory elements, and that an AT-rich core sequence (AT motif) is essential as a cis-acting element for the hepatic transcription. Homologous proteins hepatocyte nuclear factor-1 (HNF-1) , variant HNF-1 (vHNF-1) , and AT motif-binding factor 1 (ATBF1) bind to the AT motif. To clarify the role of these three different trans-acting factors, we examined the mRNA and the expression ratio of the HNF-1/vHNF-1 mRNA in various liver tissues. Our competitive reverse transcriptional polymerase chain reaction (RT-PCR) method demonstrated that this ratio is linked to their histological differentiation, and that it is higher in well-differentiated hepatocellular carcinoma (HCC) than in poorly-differentiated and undifferentiated types ; non-neoplastic liver tissues, however, have a low expression ratio of HNF-1/vHNF-1 mRNA.This study indicates that the importance of the HNF-1/vHNF-1 mRNA ratio in determining differentiation of hepatocytic neoplasms and suggests that measuring the ratio would be a useful diagnostic method. Recently, medical electronics have been developed and we must diagnose liver disease by a tiny tissues obtained from echoguided needle biopsy, however a small sized well-differentiated hepatocellular carcinoma is difficult to diagnose differentially from non-neoplastic liver tissues only by histological features. Our competitive RT-PCR method mesuring the ratio is steady and easy to obtain reliable data, using frozen tiny liver tissues, and this, in addition to histological features, must be contributing to the differential diagnosis of well-differentiated HCC from non-neoplastic lesions. We believe that the present work is highly valuable for a medical practice as well as medical science.

人白蛋白和α-竞争蛋白（AFP）基因的肝转录受一组复杂的调节元件的控制，并且在肝转录中，富含的核心序列（在基序中）作为顺式作用元素必不可少。同源蛋白肝细胞核因子-1（HNF-1），变体HNF-1（VHNF-1）和基序结合因子1（ATBF1）与AT基序结合。为了阐明这三个不同的跨作用因子的作用，我们检查了各种肝组织中HNF-1/VHNF-1 mRNA的mRNA和表达比。我们的竞争性逆转录聚合酶链反应（RT-PCR）方法表明，该比率与它们的组织学分化有关，并且在分化良好的肝细胞癌（HCC）中比差异不佳且未分化的类型更高。但是，非肿瘤肝组织的HNF-1/VHNF-1 mRNA的表达比低。这项研究表明，HNF-1/VHNF-1 mRNA比率在确定肝细胞肿瘤分化方面的重要性，并表明测量测量该比率将是一种有用的诊断方法。最近，已经开发了医疗电子产品，我们必须通过从回声的针头活检获得的微小组织来诊断肝病，但是仅通过组织学特征，很难从非肿瘤肝组织中差异诊断出一个小尺寸尺寸的良好分化的肝细胞癌。我们使用冷冻的微小肝脏组织来稳定且易于获得可靠数据的竞争性RT-PCR方法，除了组织学特征之外，这还必须有助于从非塑性病变中差异良好的HCC的差异诊断。我们认为，目前的工作对于医学实践和医学科学非常有价值。

项目成果

Hiroshi Itoh: "Intrauterine infection : Pathological study on chorioamnionitis and villitis of unknown etiology" Endocrine Journal. 41. S135-S140 (1994)

Hiroshi Itoh：“宫内感染：病因不明的绒毛膜羊膜炎和绒毛炎的病理学研究”内分泌杂志。

Hayashi Y,Ishii N,Obayashi C,Jinnai K,Hanioka K,Imai Y,Itoh H: "Thymoma, its tumorigenesis and progression in relationship with expression of EGF,EGF-Receptor, p53, v-erb B and ras p21" Virchows Archiv. (in press).

Hayashi Y、Ishii N、Obayashi C、Jinnai K、Hanioka K、Imai Y、Itoh H：“胸腺瘤，其肿瘤发生和进展与 EGF、EGF 受体、p53、v-erb B 和 ras p21 表达的关系” Virchows

Goji j, Nakamura H,Ito H,Mabuchi O,Hashimoto K,Sano K: "Expression of c-Erb B2 in human neuroblastoma tissues, adrenal medulla adjacent to tumor, and developing mouse neural crest cells" Am J Pathol. (in pree).

Goji j、Nakamura H、Ito H、Mabuchi O、Hashimoto K、Sano K：“c-Erb B2 在人神经母细胞瘤组织、邻近肿瘤的肾上腺髓质和发育中的小鼠神经嵴细胞中的表达”Am J Pathol。

Itoh H,Hanioka K,Obayashi C,Kanomata N,Itoh K,Kuroda N,Hayashi Y: "Intrauterine infection : Pathological study on chorioamnionitis and villitis of unknown etiology" Endocrine J. 41. S135-S140 (1994)

Itoh H、Hanioka K、Obayashi C、Kanomata N、Itoh K、Kuroda N、Hayashi Y：“宫内感染：病因不明的绒毛膜羊膜炎和绒毛炎的病理学研究” Endocrine J. 41. S135-S140 (1994)

Hayashi Y,Hanioka K,Kanomata N,Imai Y,Itoh H: "Clinicopathologic and molecular pathologic approaches to Lowe's syndrome" Pediatr Pathol. (in press).

Hayashi Y、Hanioka K、Kanomata N、Imai Y、Itoh H：“劳氏综合征的临床病理学和分子病理学方法”儿科病理学。