Receptors of insect neurohormones having FXPRLamide.

具有 FXPRLamide 的昆虫神经激素受体。

• 批准号: 05806007
• 负责人: MITSUI Takashi
• 金额: $ 1.22万
• 依托单位: Institute of physical & Chemical Research (RIKEN)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05806007/
• 关键词: Insect neuropeptide; Receptor; Pheromone biosynthesis; Signal transduction Silkworm; シグナル伝達; 神経ペプチドホルモン; アワヨトウ; アワヨトウ体色黒化ホルモン

To analyze the receptors of insect neurohormones having FXPRLamide at the C-terminus, we have prepared specific ligands for the receptors. Among the ligands we prepared, tritiated or iodinated probes with radio activity bound to the receptors of melanization and reddish coloration hormone (MRCH) and pheromone biosynthesis activating neuropeptide (PBAN). However, non-specific bindings to these probes were also detected. To eliminate the non-specific bindings, next we tried to design and prepared a non-radio labeled ligand having 3 functional cites (avidin binding cite, covalent bonding cite and receptor binding cite) in the peptide molecule, TKYFSPRLamide. By using this probe, we have established to detect PBAN receptor prepared from the pheromone gland of the silkworm moths.We have also elucidated the mechanism of intracellular signal transduction triggered by the binding of the neurohormone and its receptor. Our results suggested that external signal of PBAN is transduced by the influx of extracellular calcium ion, calmodulin, and phosphoprotein phosphatse which activates the final step of bombykol biosynthesis catalyzed by acyl CoA reductase.

为了分析在C末端具有FXPR酰胺的昆虫神经激素的受体，我们为受体准备了特定的配体。在我们制备的配体中，有射线活性与黑素化受体和红色色素激素（MRCH）和信息素酮生物合成激活神经肽（PBAN）结合的无线电活性。但是，还检测到与这些探针的非特异性结合。为了消除非特异性结合，我们接下来尝试设计并制备了具有3个功能性引用的非拉迪奥标记的配体（avidin结合引用，共价键合引用和受体结合引用），在肽分子Tkyfsprlamide中。通过使用此探针，我们已经建立了从蚕飞蛾的信息素腺制备的PBAN受体。我们还阐明了由神经激素及其受体的结合触发的细胞内信号转导的机制。我们的结果表明，PBAN的外部信号是通过细胞外钙离子，钙调蛋白和磷酸蛋白磷酸化的涌入来转导的，这激活了由酰基COA还原酶催化的Bombykol生物合成的最后一步。

项目成果

Matsumoto et al.: "Intracellular transduction in the regulation of pheromone biosynthesis of the silkworm, B.mori." Biosci.Biotech.Biochem.59. 560-562 (1995)

Matsumoto 等人：“细胞内转导对家蚕信息素生物合成的调节。”

Matsumoto et al.: "Multifunctionality of PBAN related neuropeptides." Proceedings of 1st International Symposium on Insect Pheromones.(in press). (1995)

Matsumoto 等人：“PBAN 相关神经肽的多功能性。”

Matsumoto et al.: "Multifunctionality of PBAN related neuropeptides." Proceedings of 1st International Symposium on Insect Pheromones. (印刷中). (1995)

Matsumoto 等人：“PBAN 相关神经肽的多功能性”。第一届国际昆虫信息素研讨会论文集（1995 年出版）。

Ozawa et al.: "Intracellular signal transduction of PBAN action in lepidopteran insects." Regul.Peptides. (in press). (1995)

Ozawa 等人：“鳞翅目昆虫中 PBAN 作用的细胞内信号转导。”

Matsumoto et al.: "Intracellular transduction of pheromone biosynthesis.." Biosci.Biotech.Biochem.59. 560-562 (1995)

Matsumoto 等人：“信息素生物合成的细胞内转导..”Biosci.Biotech.Biochem.59。