Pathogenic role of heat shock proteins gammadelta-T cells in inflammatory skin diseases

热休克蛋白γδ-T细胞在炎症性皮肤病中的致病作用

• 批准号: 04670647
• 负责人: IZAKI Seiichi
• 金额: $ 1.41万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670647/
• 关键词: heat shock protein; stress protein; HSP65; focal infection; autoimmune; mycobacterium; psoriasis; palmoplantar pustulosis; γδTリンパ球; らい

Chronic and latent infectious focus often causes or triggers the pathogenic process in distant part(s) of the body, being explained by a term "focal infection". Immunogenetically well-conserved proteins, heat shock proteins (HSPs), are inducied in cells after exposure to elevated temperature or others. This phenomenon has been investigated in relation to both host-defense to dacteria and induction of autoimmunity, since bacterial HSPs are immunogenic and targetted by immune surveilance, while mammalian HSPs induced after infectious stress are able to be recognized by the same immune mechanism. We started to investigate antibody production to mycobacterial HSP65 in various inflammatory skin diseases in order to correlate this so-called 'focal infection".In 1992, we obtained data from approximately 200 patients with various skin diseases. In comparison to normal group, patients with palmoplantar pustulosis (PPP), psoriasis (Pso), urticaria, and herpes zoster frequently showed elevated Ig … More G antibody to HSP65 from Mycobacterium leprae assayd on ELISA plates. In patients with anaphylactoid purpura the antibody level was not raised.In 1993, we further investigated correlation between antibody to HSP65 and skin inflammatory diseases triggered by bacterial infection. Patients with PPP were newly collected and divided into groups with and without chronic tonsilitis or periodontitis as infectious focus. OD at 492 nm in this ELISA technique revealed significant (p<0.01) difference between PPP with infectious focus (n=7,0.230(〕SY.+-.〔)0.065, mean(〕SY.+-.〔)SD) and PPP without it (n=12,0.139(〕SY.+-.〔)0.066). IN psoriasis, collected patients were divided into three groups according to types of psoriatic lesions : acute guttate psoriasis (PGA) that is a particular type of psoriasis induced often after steptococcal infection, plaque-type psoriasis (PV) and psutular-form psoasis (PP). The anti-HSP65IgG levels were 0.174(〕SY.+-.〔)0.032 for PGA (n=4), 0.101(〕SY.+-.〔)0.053 for PV (n=12) and 0.087(〕SY.+-.〔)0.025 for PP (n=6), showing significant changes between PGA and others (p=0.0165, PGA - PV ; p=0.001, PGA - PP).Elevation of anti-HSP65IgG was found in relation to bacterial infection-induced psoriatic and leukotactic inflammation in the skin. Less

慢性和潜在的感染焦点通常会引起或触发人体遥远部分的致病过程，并由“局部感染”一词解释。在暴露于温度升高或其他人后，在细胞中诱导了免疫力保存良好的蛋白质，热休克蛋白（HSP）。由于细菌HSP具有免疫原性，并且通过免疫监测靶向，而在感染胁迫后诱导的哺乳动物HSP可以通过相同的免疫力学识别，因此已经研究了这种现象与宿主防御和自身免疫性有关的宿主防御和诱导自身免疫性。我们开始研究各种炎症性皮肤疾病中对分枝杆菌HSP65的抗体产生，以便将这种所谓的“局灶性感染”相关。在ELISA平板上测定的HSP65抗体。感染性重点。在牛皮癣中，根据银屑病病变的类型将收集的患者分为三组：急性肠肠牛皮癣（PGA），这是在继发性感染后经常引起的一种特定类型的牛皮癣，斑块型银屑病（PV）和庞然大物 - 庞然大物（PP）。 PGA（n = 4），0.101（>SY。+ - 。>）0.032的抗HSP65igG水平为0.174（> SY。+ - 。>）0.032，PV（n = 12）和0.087（>SY..ISSE.。+ - 。>。 p = 0.001，pga-pp）。在皮肤感染诱导的牛皮癣和白细胞术中发现了抗HSP65IGG的抗抗HSP65IGG。较少的

项目成果

Yoshizawa Y,Shinozaki Y,Izaki S,Kitamura K.: "Hemostatic and fibrinolytic changes in herpes zoster.In:Current Aspects of Blood Coagulation,Fibrinolysis and Platelets.ed by Schen M-C,Teng C-M,Takada A" Springer-Verlag,Tokyo., 88-92 (1993)

Yoshizawa Y、Shinozaki Y、Izaki S、Kitamura K.：“带状疱疹的止血和纤溶变化。见：血液凝固、纤溶和血小板的当前方面。由 Schen M-C、Teng C-M、Takada A 编辑”Springer-Verlag，东京

伊崎 誠一: "線溶系の新展開:細胞機能に関与する線溶系因子の役割" 皮膚の機能と線溶系因子, 79-93 (1992)

Seiichi Izaki：“纤溶系统的新进展：纤溶因子在细胞功能中的作用”皮肤功能和纤溶因子，79-93（1992）

Izaki S,Goto Y,Kitamura K,Nomaguchi H: "Antibody to 65-kD stress protein(HSP65)of Mycobacterium leprae invarious inflammatory skin diseases.A preliminary report." Jpn J Leprosy. 63(in press). (1994)

Izaki S、Goto Y、Kitamura K、Nomaguchi H：“针对各种炎症性皮肤病的麻风分枝杆菌 65-kD 应激蛋白 (HSP65) 的抗体。初步报告。”

飯塚 万利子,伊崎 誠一,北村 啓次郎,丸山 征郎: "血管系腫瘍のマーカーとしてのトロンボモジュリンの意義" 皮膚科の臨床. 35. 411-415 (1993)

Mariko Iizuka、Seiichi Izaki、Keijiro Kitamura、Seiro Maruyama：“血栓调节蛋白作为血管肿瘤标志物的意义”《临床皮肤病学》35. 411-415 (1993)。

Izaki S,Hirai A,Yoshizawa Y,Kitamura K,Inoue T,Hatoko M,Itoyama S,Inazu M.: "Carcinosarcoma of the skin: immunohistochemical and electron microscopic characterization." J Cutan Pathol. 20(2). 272-278 (1993)

Izaki S、Hirai A、Yoshizawa Y、Kitamura K、Inoue T、Hatoko M、Itoyama S、Inazu M.：“皮肤癌肉瘤：免疫组织化学和电子显微镜表征。”