Study of the biochemical processing of the biowarning information through the hypothalamu

下丘脑生物预警信息生化处理的研究

基本信息

批准号：
04670095
负责人：
SHIMIZU Nobuaki
金额：
$ 1.34万
依托单位：
Kanazawa University (1993)Kyushu University (1992)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

This study was designed to study the dynamic changes in serotonin (5HT) and noradrenaline (NA) levels during the stressful stimuli, and have reached the following conclusions in rats. (1) Immobilization-stress caused anorexia in conjunction with an immediate increase in thehypothalamic 5HT release. Injection of 5HT receptor antagonist significantly reduced the immobilization-induced anorexia, suggesting that the hypothalamic serotonergic ystem is activated during stress and 5HT is an inhibitory transmitter in feeding behavior. (2) Immobilization elevated NA levels in the medial prefrontal cortex (PFC). Pre-injection of a benzodiazepine receptor agonist, diazepam, significantly attenuated the immobilization-induced NA release, while the injection of an anxiogenic compound, FG7142, facilitated it in non-stressed condition. Thus the stressfull stimuli activate the NA system in the medial PFC which may be modulated by an activation of the benzodiazepine receptors in the brain. (3) Immobilization-stress rapidly increased NA levels of spleen in conjunction with the suppression of natural killer (NK) cytotoxicity. Both theincreased levelsof NA ad he suppresion of NK cytotoxicity were significantly attenuated after surgical denervation of the splenic sympathetic nerve, indicating that immobilization-induced suppression of splenic N/K cytotoxicity was mediated predominantly through enhanced activity of the splenic sympathetic nerve..These results suggest that both 5HT and NA may act asneurotransmitters and/or neuromodlators which regulate biowarning processes in the living body.

这项研究旨在研究压力性刺激期间5-羟色胺（5HT）和去甲肾上腺素（NA）水平的动态变化，并在大鼠中得出了以下结论。（1）固定的压力与高丘脑5HT释放的立即增加引起厌食症。注射5HT受体拮抗剂可显着降低固定化诱导的厌食症，这表明下丘脑血清素能在压力期间被激活，而5HT是进食行为中的抑制性递质。（2）内侧前额叶皮层（PFC）中固定的Na水平升高。预注射苯二氮卓受体激动剂地西ep剂，显着减弱了固定化诱导的NA释放，而注射焦虑化合物FG7142则在无压力条件下促进了它。因此，应激刺激激活了内侧PFC中的Na系统，这可能是通过大脑中苯二氮卓受体的激活来调节的。（3）与抑制天然杀手（NK）细胞毒性结合使用，固定压力迅速增加了脾脏水平。在手术对脾交感神经的手术区域进行手术区域后，促进NK细胞毒性的水平都显着减弱，表明固定诱导的脾脏N/K细胞毒性抑制了脾脏的抑制，主要是通过脾脏的同情性神经的增强来介导的。调节生物体内生物载体过程的神经调节剂。