Targeted sonocatalytic cancer cell injury using avidin-conjugated titanium dioxide nanoparticles

使用亲和素缀合的二氧化钛纳米粒子靶向声催化癌细胞损伤

• 批准号: 24650294
• 负责人: SHIMIZU Nobuaki
• 金额: $ 2.5万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650294/
• 关键词: 超音波; 二酸化チタン; ナノ粒子; OHラジカル; がん幹細胞

In this study, we applied sonodynamic therapy to cancer cells based on the delivery of titanium dioxide (TiO2) nanoparticles (NPs) modified with avidin protein, which preferentially discriminated cancerous cells from healthy cells. Subsequently, hydroxyl radicals were generated from the TiO2 NPs after activation by external ultrasound irradiation (TiO2/US treatment). Although 30% of the normal breast cells (human mammary epithelial cells) exhibited the uptake of avidin-modified TiO2 NPs, over 80% of the breast cancer cells (MCF-7) exhibited the uptake of avidin-TiO2 NPs. Next the effect of the TiO2/US treatment on MCF-7 cell growth was examined for up to 96 h after 1-MHz ultrasound was applied (0.1 W/cm2, 30 s) to cells that incorporated the TiO2 NPs. No apparent cell injury was observed until 24 h after the treatment, but the viable cell concentration declined to 68% compared with the control at 96 h.

在这项研究中，我们根据用抗多素蛋白修饰的二氧化钛（TIO2）纳米颗粒（NPS）的递送对癌细胞应用了声动力疗法，该纳米颗粒（NPS）优先区分了健康细胞的癌细胞。随后，通过外部超声辐射激活（TIO2/US处理），从TIO2 NP产生羟基自由基。尽管有30％的正常乳腺细胞（人类乳腺上皮细胞）表现出甲虫改性的TiO2 NP的摄取，但超过80％的乳腺癌细胞（MCF-7）表现出对Avidin-Tio2 NP的摄取。接下来，在1 MHz超声（0.1 w/cm2，30 s）应用于掺入TIO2 NP的细胞后，检查了TIO2/US处理对MCF-7细胞生长的影响96小时。直到治疗后24小时才观察到明显的细胞损伤，但是与对照组相比，活细胞浓度下降至68％。

项目成果

微生物をキャリアとした遺伝子導入法における超音波照射の効果

以微生物为载体的基因转移方法中超声波照射的效果

• 发表时间: 2013
• 作者: 仁宮一章;山田龍治;清水宣明
• 通讯作者: 清水宣明

Inactivation of Escherichia coli by sonoelectrocatalytic disinfection using TiO2 as electrode

TiO2 电极声电催化消毒灭活大肠杆菌

• 发表时间: 2013
• 期刊: Ultrason. Sonochem.
• 作者: Kazuaki Ninomiya;Masayuki Arakawa;Chiaki Ogino;and Nobuaki Shimizu
• 通讯作者: and Nobuaki Shimizu

Image analyzing method to evaluate in situ bioluminescence from an obligate anaerobe cultivated under various dissolved oxygen concentrations

• DOI: 10.1016/j.jbiosc.2012.09.006
• 发表时间: 2013-02-01
• 期刊: JOURNAL OF BIOSCIENCE AND BIOENGINEERING
• 影响因子: 2.8
• 作者: Ninomiya, Kazuaki;Yamada, Ryuji;Shimizu, Nobuaki
• 通讯作者: Shimizu, Nobuaki

超音波照射による二酸化チタンプレート表面での細胞傷害効果

超声波照射对二氧化钛板表面的细胞毒性作用

• 发表时间: 2013
• 作者: 丸山裕慎;仁宮一章;清水宣明
• 通讯作者: 清水宣明

Cell-SELEX based selection and characterization of DNA aptamer recognizing human hepatocarcinoma

• DOI: 10.1016/j.bmcl.2013.01.040
• 发表时间: 2013-03-15
• 期刊: BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
• 影响因子: 2.7
• 作者: Ninomiya, Kazuaki;Kaneda, Kazuhiko;Shimizu, Nobuaki
• 通讯作者: Shimizu, Nobuaki