Development of a novel micropropagation system in Lilium longiflorum by film culture vessel

薄膜培养皿开发长花百合新型微繁殖系统

• 批准号: 04660035
• 负责人: TANAKA Michio
• 金额: $ 1.09万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660035/
• 关键词: Lilium longiflorum; Micropropagation; Film cullure vessel; Bulblet production

The bulblet production from Lilium longiflorum scales cultured in vitro was studied by using a novel disposable envelope-shaped culture vessel, the "Culture Bag" made of fluorocarbon polymer films (Neofron (〕SY.encircledR.〔) films). This paper dealt with the bulblet formation in scales cultured in vitro on modified Murashige and Skoog (MS) agarmedium (half macro-and micro-nutrients, 4.5% sucrose) in flask and in the liquid medium in the Culture Bag (90 x 40 mm) made of Neoflon FEP or PFA film of 12.5, 25, 50, and 100 mum thickness under light or dark condition. In both culture vessels, larger bulblets and higher number of bulblets per scale were obtained under light than dark. The number of bulblets per scale and fresh weight of bulbrets in any of the Culuture Bag were in fact higher when compared to those in flask. The number of foliage scales formed on bulbrets obtained in the Culuture Bag made of FEP film was higher than those in the Culuture Bag were in fact higher when compared to … More those in flask. The number of foliage scales formed on bulblets obtained in the Culture Bag made of FEP film was higher than those in the Culuture Bag made of PFA film. Further work showed that much larger bulblets were obtained in MS (full-strength nutrients, 4.5% sucrose) liquid medium in the Culture Bag made of PFA film compared to those on the agar medium in flask. An attempt was also made to develop the svale-up systems by using the Culuture Bag which is nine times larger (270 x 120 mm) than the smaller size and the 4-chambered Culuture Bag of larger size.the possible advatages to using the Culuture Bag system in the micropropagation of Lilium are as follows ; (1) bigger bulblets with very short foliage leaves and roots could be obtained than those in the conventional micropropagation, (2) these bulblets could be saved when inoculating explants to the large size Culuture Bag, and (4) the cost for the production of bulblets is expected to be much reduced by this novel system when vompared to those by the fermentor system and the tank culture system. Less

采用新型一次性信封状培养容器，即由氟碳聚合物薄膜（Neofron (〕SY.encircledR.〔) 薄膜）制成的“培养袋”，对体外培养的百合鳞片的生产进行了研究。在改良的 Murashige 和 Skoog (MS) 藻培养基（一半常量和微量营养素，4.5%）上体外培养的鳞片中的鳞茎形成在光照或黑暗条件下，在烧瓶中和由 Neoflon FEP 或 PFA 薄膜制成的培养袋（90 x 40 mm）中的液体培养基中，厚度为 12.5、25、50 和 100 μm。与黑暗相比，每个鳞片的鳞茎数量都更高。实际上，任何培养袋中的每个鳞片的鳞茎数量和鳞茎鲜重都更高。由 FEP 薄膜制成的培养袋中的小鳞茎上形成的叶鳞数量实际上比培养袋中的小鳞茎上形成的叶鳞数量要高。 FEP 膜制成的培养袋中获得的球茎含量高于 PFA 膜制成的培养袋中的球茎含量（全强度养分，4.5%）。与烧瓶中的琼脂培养基相比，PFA 膜制成的培养袋中的琼脂培养基（蔗糖）液体培养基还尝试使用大九倍的培养袋（270 x 120 毫米）开发 svale-up 系统。 (1)大于较小尺寸和较大尺寸的4室Cululure Bag。与传统微繁殖相比，可以获得具有非常短的叶和根的小鳞茎，（2）当将外植体接种到大尺寸培养袋中时可以节省这些小鳞茎，以及（4）预期小鳞茎的生产成本与发酵罐系统和罐培养系统相比，这种新颖的系统大大减少了。