Biological Function of Retinoic Acid Receptors in Bronchopulmonary System

支气管肺系统视黄酸受体的生物学功能

基本信息

批准号：
03807046
负责人：
AKITA Hirotoshi
金额：
$ 1.22万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807046/
关键词：
Lung Cancer Retinoic Acid Retinoic Acid Receptor レチノイン酸リセプタ-

项目摘要

Purpose: In this study, the expression of retinoic acid receptor(RAR) alpha and beta was examined in order to understand the role of retinoic acid(RA) in the development and function of bronchopulmonary system.Result: RARalpha was shown to be expressed in human adult lung, and rat adult and newborn lung in Northern blot analysis. This data suggested the important role of RA and RARalpha in the development and function of bronchopulmonary system. On the other hand, the expression of RARbeta gene was not detected in human adult lung, and rat newborn and adult lung in Northern blot analysis. This data suggested the tissue-soecific expression of RARbeta gene. Expression and structural abnormality of RARbeta gene was studied in human lung cancer cell lines using RNAse protection assay, since RARbeta gene was known to be located in the short arm of chromosome 3. No expression of RARbeta gene was detected in human lung cancer cell lines. This finding suggested that the loss of the RARbeta expression could be a very important feature in the process of carcinogenesis and progression of human lung cancer.In the future, it is important show the possibility of the clinical application of the RA-RAR system by studying whether it is possible to inhibit the transformation of cells with RA and/or RAR, and whether it is possible to reverse the transformed phenotype of cancer cells with RA and/or RAR.

目的：本研究检测视黄酸受体（RAR）α和β的表达，以了解视黄酸（RA）在支气管肺系统发育和功能中的作用。结果：RARα在支气管肺系统的发育和功能中表达。 Northern 印迹分析中的人类成人肺、大鼠成人和新生儿肺。该数据表明 RA 和 RARα 在支气管肺系统的发育和功能中的重要作用。另一方面，在Northern印迹分析中，在人成人肺、大鼠新生儿和成人肺中未检测到RARbeta基因的表达。该数据表明 RARbeta 基因的组织特异性表达。由于已知RARbeta基因位于3号染色体短臂，因此利用RNA酶保护实验研究了RARbeta基因在人肺癌细胞系中的表达和结构异常。在人肺癌细胞系中未检测到RARbeta基因的表达。这一发现提示RARbeta表达的缺失可能是人类肺癌发生、发展过程中一个非常重要的特征。未来通过研究揭示RA-RAR系统临床应用的可能性具有重要意义。是否可以用RA和/或RAR抑制细胞的转化，以及是否可以用RA和/或RAR逆转癌细胞的转化表型。