Clarification of etiology of neonate and infant vitamin K deficiency

澄清新生儿和婴儿维生素 K 缺乏症的病因

基本信息

批准号：
62480173
负责人：
KIMURA Mieko
金额：
$ 0.32万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480173/
关键词：
vitamin K vitamin K deficiency neonate infant determination method for vitamin K platinum Column plasma umbilical cord plasma breast-milk qmniotic membrane amniotic fluid meconium ミネラル類新生児・乳児ビタミンK定量法母体血清臍帶血清胎盤

项目摘要

It is well known that neonatal and infantile vitamin K deficiency causes melena neonatorumand intracranial hemorrhagic disorders. These disorders occur more frequently in breast-fed babies than in bottle fed babies, but the reason is still obscure. There have been some suggestions as to how to prevent babies from having vitamin K deficiency. On the other hand, determination of endogenous concentrations of the K vitamins (phylloquinone:K1 and menaquinones:MK-4, MK-l, MK-7)in human plasma is very difficult. In this study, at first we report clear separations and determination methods of vitamins K1 and MK-4, MK-l and MK-7 in placenta with the use of thin layer chromatography, followed by high performance liquid chromatography(HPLC) and fluorometric determination after post-column chemical reduction, in maternal and umbilical cord plasma by HPLC and fluorometric determination follwed post-column electochemical reduction in maternal and umnilical cord plasma by HPLC and fluorometric determ … More ination follwed platinum reduction column. Next, we describe the availability of vitamin Ks in different tissues. Vitamins K1 and MK-4, MK-6 and MK-7 were found in placenta, and maternal plasma, much K1 and MK-48 and slightly MK-l and MK-7 were found in breast milk and amniotic membrance, and K1 and MK-4 were found in umbilical cord plasma, smniotic luid, meconium and vernix caseosa. Third, to prevent early hemorrhagic diseases of the newborn, we have administered vitamin K2 drug by i.v. or oral route to mother before delivery, and vtaimin Ks concentrations in umbilival cord plasma, maternal plasma at birth and breast milk of 5 days after delivery were found to be siginficantly high as ccompared with those of non-treated mothers group. Therefore, oral or i.v. administration of vitamin Ks drug to mother before delivery which causes early and continuos transportation of vitamin Ks from mother to neonate is safer and more preferable than intramusclar injection to neonate, whic involves a certain degree of danger. Less

众所周知，新生儿和婴儿维生素 K 缺乏会导致新生儿黑便和颅内出血性疾病，这些疾病在母乳喂养的婴儿中比在奶瓶喂养的婴儿中更常见，但其原因仍然不清楚。预防婴儿维生素 K 缺乏另一方面，测定维生素 K 的内源浓度（叶绿醌：K1 和甲基萘醌：MK-4、MK-l、人血浆中维生素K-7)的检测是非常困难的，本研究首先报道了胎盘中维生素K1和MK-4、MK-1和MK-7的清晰分离和测定方法，随后采用薄层色谱法进行分离和测定。柱后化学还原后通过高效液相色谱 (HPLC) 和荧光测定，在母体和脐带血浆中通过柱后电化学还原后通过 HPLC 和荧光测定接下来，我们描述了在胎盘中发现的不同组织中维生素 K1 和 MK-4、MK-6 和 MK-7 的可用性。和母体血浆中，母乳和羊膜中发现大量 K1 和 MK-48，少量 MK-l 和 MK-7，K1 和 MK-4第三，为了预防新生儿早期出血性疾病，我们在分娩前通过静脉注射或口服途径给母亲注射维生素 K2 药物，以提高脐带血浆中的维生素 K2 浓度。与未接受治疗的母亲组相比，母亲出生时的血浆和产后 5 天的母乳显着升高。因此，在分娩前给母亲口服或静脉注射维生素K药物，使维生素K从母亲早期持续输送到新生儿，比肌肉注射给新生儿更安全、更可取，但危险性较小。