Localization and Kinetics of Neural Transmitters in the Enteric Nervous System. Studies Using Laser Photoooagulations and Immunochemistry.

肠神经系统中神经递质的定位和动力学。

基本信息

批准号：
62480093
负责人：
KOBAYASHI Shigeru
金额：
$ 4.29万
依托单位：
Yamanashi Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

We investigated nerve regeneration. following lesion forming Argon ion laser irradiation in the guinea-pig small intestine by immunohistochemistry using peroxidase-antiperoxidase complex techniques over a time course of up to 70 days.Round laser-lesions about 0.5mm in diameter were produced and the regenerative features of the two major cell types of the enteric nerve plexuses. i.e. enteric neurons containing neuropeptide by such as methionine-enkephalin-Arg^6-Gly^7-Leu^8 (Enk^<-8>).Peptide-hisbydine-isoleucine (PIII). substance P (SP) and somatostatin (SM) together with tby enteric glial cells containing nerve tissue protein S 100b (S-100b protein by were examined.Immediately after the laser irradiation. the histological structure of the enteric nerve plexuses remained almost intact. though both neuronal and glial cells were coagulated and dead. The immunoreactivities for neuropeptides and S-100b proteins in the neuronal and glial elements respectively were also mostly preserved. By 3 … More hrs after the laser irradiatio, a conspicuous accumulation of immunoreactivities to neuropeptides occurred in the severed nerve stumps. The cellular debris, containing neuropeptides/S-100b protein, were gradually removed from the lesion by 3 days. Since neuropeptides in the neuronal processes are transported from the cell body by the fast anterograde axonal flow, an early accumulation of neuropeptides on the oral edge of the lesion indicates that the neurons project processes in the oro-anal direction, and vice versa.It was deduced that, in the myenteric plexus, both Enk-8 and SP neurons issued both orally and anally directed processes; whereas, PHI and SM neurons sent processes mainly in the oro-anal direct-ion. In the deep muscular plexus (DMP), Enk-8 and SP neurons ran circularly. In the submucous plexus, projections of PHI, SP and SM neurons were directed evenly in all directions.Neuropeptide immunoreactivities of the axotomized nerve cell bodies in the area surrounding the lesion became strikingly stronger 3 to 10 days after laser irradiation. This phenomenon was interpreted as a mode of retrograde degeneration of the enteric neurons. At 15 to 70 days, regenerated nerve plekuses completely spread over the scar tissue of the laser lesion. No nerve cell body, however, existed in the lesioned ganglia. In the regenerated nerve plexus, the glial cells, which proliferated by mitosis, supported and guided the nerve fibers which extended from the survived neuronal cell bodies in the ganglia around the lesion. This suggests that the characteristic neuron/glial cell interactions in the laser lesion might also occur daring the continuous remodeling of the autonomic groundplexus under non-experimental conditions in the enteric nerve plexuses. Less

我们研究了神经再生。在病变形成几内亚小肠中通过免疫组织化学形成氩离子激光照射的，在长达70天的时间过程中，使用过氧化物酶 - 抗氧化酶复合酶复杂技术进行了。直径约0.5mm的螺旋激光质量是直径约0.5mm的。即含有神经肽的肠神经元，例如甲基氨酸 - 源代源性^6-gly^7- leu^8（enk^<-8>）。肽 - 二甲状腺素 - 异丙胺（PIII）。物质P（SP）和生长抑素（SM）以及含有神经组织蛋白S 100B（检查S-100B蛋白的TBY肠神经胶质细胞（检查S-100B蛋白）。在激光照射后发生。肠神经丛的组织学结构几乎是完整的。神经元和神经元的元素也大多保留在激光辐射后的3个。过程是通过快速的顺序轴突流从细胞体传输的，神经肽在病变的口腔边缘的早期积累表明神经元在Oro-Anal方向上进行过程，反之亦然。而PHI和SM神经元主要在Oro-Anal Direct-Ion中发送过程。在深肌丛（DMP）中，ENK-8和SP神经元在粘膜丛中运行，PHI，SP和SM神经元的项目均在各个方向上均匀地定向。神经肽免疫反应性的轴向神经细胞的免疫反应能力使周围地区周围地区的轴向神经细胞变得非常强大3至10天。这种现象被解释为肠神经元的逆行变性方式。在15到70天时，再生神经胸膜完全分布在激光病变的疤痕组织上。然而，病变的神经节中没有神经细胞体。在再生神经丛中，通过有丝分裂增殖的神经胶质细胞支持并引导神经纤维，这些神经纤维是从生存的神经元中围绕病变周围神经节中延伸的神经纤维。这表明激光病变中的特征性神经元/神经胶细胞相互作用也可能会发生在Enter神经丛中的非实验条件下自主地面的连续重塑。较少的