Analysis of the genes in lymphocyte differentiation.

淋巴细胞分化基因分析。

基本信息

批准号：
61480478
负责人：
KUROSAWA Yoshikazu
金额：
$ 4.42万
依托单位：
FUJITA-GAKUEN HEALTH UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

We are analyzing functions of sigma structure in human immunoglobulin heavy chain gene loci: _ located downstream of enhancer, _ and _ located between and genes, _ located upstream of s_ . In this year, we carried out the following experiments.1)We isolated immunoglobulin gene from DNA of the insectivors suncus murinus, and determined its nucleotide sequence. Comparison of nucleotide sequences between man mouse and suncus will give us information for biologically meaningful regions.2. We established a B cell line expressing both IgM and IgG on the cell surface by transformation of peripheral plood cells of a patient with hyper IgM immunodeficiency using epstein-barr viruses. The data showed that in double isotype-bearing cells, one VHDHJH exon in this transript is alternatively spliced to C_ or C_ without DNA rearrangement. The defect in this disease could be at the S-S recombination stage.3) Although the frequency of IgD-secreting cells is extremely low in mouse, about 1% of the patients with myeloma produce IgD in humam. We analyzed dna from human IgD myelomas. The data indicated that homologous recombination between _ and _ sequences mediates class switch from to in human, and that it occurs via unequal crossing over between sister chromatids or daughter chromosomes.4)We are analyzing mechanism alternative rna splicing in ^- and ^- double producers.5)We are analyzing precence of discontinuous transcription followed by trans RNA splicing.

我们正在分析人类免疫球蛋白重链基因基因座中的Sigma结构的功能：_位于增强剂的下游，_和_位于S_的上游，位于_之间和基因之间。在今年，我们进行了以下实验。1）我们从植物素的DNA中分离了免疫球蛋白基因，并确定了其核苷酸序列。对男子小鼠和日气之间的核苷酸序列的比较将为我们提供有关生物学上有意义的区域的信息。2。我们通过使用爱泼斯坦 - 巴尔病毒的超级IGM免疫缺陷患者的外周绒毛细胞的转化，建立了在细胞表面上同时表达IgM和IgG的B细胞系。数据表明，在双同种型细胞中，该转移中的一个VHDHJH外显子替代地剪接至C_或C_，而无需DNA重排。 The defect in this disease could be at the S-S recombination stage.3) Although the frequency of IgD-secreting cells is extremely low in mouse, about 1% of the patients with myeloma produce IgD in humam.我们分析了人类IgD骨髓瘤的DNA。数据表明，_和_序列之间的同源重组介导了类从人类转变为人类，并且它是通过在姐妹染色单体或女儿染色体之间跨越不平等的跨越。