Construction and expression of gene families

基因家族的构建和表达

• 批准号: 03454561
• 负责人: KUROSAWA Yoshikazu
• 金额: $ 4.1万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454561/
• 关键词: MHC; Complement; Immune system; Homeobox; Sponge; 抗体; 円口類; 補体第二経路; 進化

It has been believed that the most primitive animal that produces antibodies is cyclostome. We isolated the gene which encodes hagfish "antibodies" and determined the nucleotide sequence. Surprisingly, it turned out to be a gene encoding not immunoglobulin but complement C3. We developed a new method using PCR for isolation of MHC genes from primitive vertebrates. By this method, we have succeeded to isolate genes encoding MHC classI and classIIbeta from carp and also a gene encoding MHC classI from shark. We also showed that at the level of bony fish MHC classI genes have been expanded to at least several tens and their sequences are quite heterogeneous.We examined homeobox-containing genes from various phyla of animals. We isolated two homeobox genes, prox1 and prox2 from sponge. The structure of prox1 and prox2 indicated that they have gained the properties characteristics for each class of homeobox genes isolated from higher animals. We isolated four homeobox genes from hydra. A new kind of homeobox gene was identified from C. elegans. Two kinds of new homeobox genes, GBX1 and GBX2, were isolated from human. We mapped GBX1 to chromosome 7q36.1.

人们认为，产生抗体的最原始动物是循环仪。我们分离了编码hag鱼“抗体”并确定核苷酸序列的基因。令人惊讶的是，事实证明它是编码不是免疫球蛋白而是补充C3的基因。我们使用PCR开发了一种新方法来分离原始脊椎动物的MHC基因。通过这种方法，我们成功地将编码MHC Classi和Classiibeta的基因与鲤鱼的基因进行了分离，也可以通过鲨鱼编码MHC Classi的基因。我们还表明，在骨鱼MHC的水平上，MHC分类基因已扩展到至少数十个，它们的序列非常异质。我们检查了来自各种动物门的含同基因的基因。我们从海绵中分离了两个同源基因Prox1和Prox2。 Prox1和Prox2的结构表明，它们已经获得了从上等动物分离的每类同源基因的特性特征。我们从Hydra分离了四个同源基因。从秀丽隐杆线虫中发现了一种新型同源基因。从人类中分离出两种新的同源基因GBX1和GBX2。我们将GBX1映射到7q36.1染色体。

项目成果

M.Naito: "Identification of a homebox-containing gene between lin-45 and unc-24 at chromosome IV in the nematode Caenorhabditis elegans." Nuc.Acids Res.

M.Naito：“在线虫秀丽隐杆线虫第四号染色体 lin-45 和 unc-24 之间含有同源盒的基因的鉴定。”

K. Okamura: "Expansion of genes that encode major histocompatibility complex class I-like moleucles in cyprinid fishes." Immunology.

K. Okamura：“鲤鱼中编码主要组织相容性复合物 I 类分子的基因的扩展。”

K.Hashimoto: "Identification of a shark sequence resembling the major histocompatibility complex class I α3 domain." Proc.Natl.Acad.Sci.USA.

K.Hashimoto：“重新组装主要组织相容性复合体 I 类 α3 结构域的鲨鱼序列的鉴定。”Proc.Natl.Acad.Sci.USA。

T.Matsui: "Expression of a novel human homeobox-containing gene mapped to chromosome 7q36.1 in hematopoictic cclls."

T.Matsui：“在造血细胞中映射到染色体 7q36.1 的一种新型人类同源盒基因的表达。”

K.Okamura: "Expansion of genes that encode major histocompatibility complex classI-like molecules in cyprinid fishes." J.Immunology.

K.Okamura：“鲤鱼中编码主要组织相容性复合物 I 类分子的基因的扩展。”