The properties and regulations of proteins to form cytoskeletons in higher plants

高等植物细胞骨架蛋白质的性质和调控

基本信息

批准号：
61480012
负责人：
MIZUNO Koichi
金额：
$ 4.03万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

We have studied about the properties and functions of cytoskeletal elements and its protein compositions in higher plants.Cultured tobacco cells possess minor tubulin-like protein,which is immunologically classified as <alpha>-tubulin. This protein induced the depolymerization and the inhibition of polymerization of microtubules.Immunofluorescent studies using antibodies against tubulin often showed the microtubules protruding from the surface of nuclei to cytoplasm and sometimes to inside of nuclei just like the initiation of spindle microtubules formation.This observation suggested the localization of MTOC-like substances on nuclei.In fact,MTOC-like active substance(s)was prepared from nuclei of higher plants.In vitro polymerizable actin was isolated from hifer plant cells.The biochemical properties would be shown within short time.We found three novel cytoskeletal elements from higher plant cells.They seems to be unique cytoskeletons in higher plants,therefore,the regulation systems … More will be interest.50 kd protein polymerizad intermediate filament-like structures in the presence of guanosine nucleotides.This protein was shown to be basic protein and cross-reacted with antitubulin antibodies.Monoclonal antibodies showed the localization of 50 kd protein around the nuclei and cortical region as network in interphase cells.Phragmoplast was also shown to contain this protein.58 kd protein was purified from insoluble fraction in the presence of ATP,this protein readily solubilized by the depletion of ATP.Addition of ATP induced the reversible polymerization and formed fibrillar bundles of thin filament having a diameter about 3[5 nm.Monoclonal antibodies showed the distribution of this bundles around the surface of nuclei protruding to plasma membrane. Possible involvement of the fibrillar bundles in the mechanism to determine the site at which cytokinesis will occur and to move the nucleus in relation to the division site.100 kd protein was solubilized with KC1 fron insoluble fraction and purified.This protein polymerized double chain filaments haying a diameter about 15 nm in vitro.The function of these cytoskeletal element should be further studied. Less

我们已经研究了细胞骨架元素及其蛋白质组成在高等植物中的特性和功能。培养的烟草细胞具有较小的微管蛋白样蛋白，该蛋白质被免疫学分类为<Alpha> - 细胞蛋白。该蛋白诱导了使用抗微管蛋白的抗体的免疫荧光研究引起的沉积和抑制，通常表明从核表面到细胞质向细胞质的微管蛋白突出，有时甚至是核内部的微管内部，就像固定型的定位。事实，从核电站制备了MTOC样活性物质。从Hifer植物细胞中分离出可分离的肌动蛋白。将在短时间内显示生化特性。我们发现三个新的细胞骨骼元素来自较高的植物细胞的三个新型细胞骨骼元素，它们似乎是较高的植物中的独特细胞骨骼，较高的蛋白酶属性，属于sofers firers.50 kd kd kd。在存在鸟嘌呤核动肽的情况下。该蛋白显示为碱性蛋白质，并与抗维蛋白抗体进行交叉反应。单克抗体表明，在核和皮质区域围绕网络作为网络中的50 kD蛋白在相间细胞中的核和皮质区域的定位也表明了该蛋白质的蛋白质。 ATP的部署很容易溶于蛋白质。ATP的成立诱导了可逆的聚合，并形成直径约为3的薄丝的纤维束[5 nm。单克隆抗体表明了该捆队周围围绕Plasmambrane的核表面周围的捆绑链分布。原纤维束可能参与该机制，以确定将发生细胞因子的位点并移动与分裂位点相关的核素。100kD蛋白可溶于KC1前不溶性馏分并纯化并纯化。该蛋白质蛋白质聚合的双链链链含量约为15 nm nm nm ym intro e元素。较少的