Capillary Tube Isotachophoresis Using Ligand-replacement Equilibria in Dipole Aprotic Solvent.

在偶极非质子溶剂中使用配体置换平衡的毛细管等速电泳。

基本信息

批准号：
61470032
负责人：
HITOSHI YOSHIDA
金额：
$ 3.97万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

The development of new migrating systems was studied to enhance the sensitivity and the separability of the capillary tube isotachophoresis(CTIP) in non-aqueous, mixed and aqueous solvent.1. New migrating systems, whose terminating ion played an important role to control the effective mobility of sample species, were developed by using aprotic solvent, dimethylformamide. In this systems, chloride of terminating ion acts as complexing agent, so the heavy metal ions injected are converted into the chloro-complexes. The effective mobilities of each chloro-complex were controled by complexing forming equilibria with terminating ion and the separation of the heavy metal ions could be achieved. Using cyanide or EDTA as terminating ion, heavy metal ions and rare earth metal ions were separated in aqueous and mixed solvent, respectivety. Further, the migrating system with borate as terminating ion could be applicable to the separation of electric neutral species such as catechol derivatives.2. … More The migration systems to combine CTIP with pre-concentration were developed to enhance the sensitivity of CTIP. When HCIO_4-HCI migrating systems with dimethylformamide as solvent was used, organic solvent containing DDTC chelates of heavy metal ions after extraction could be directry injected into the migration column and these chelates were converted into the corresponding anionic chloro-complexes by the ligand substitution reaction with terminating ion,CI^-. Consequentiy, the metal chelates could be migrated as the chloro-complexes and separated sensitivity. Also both the oxine and dithizone chelates of heavy metal ions could be applicable in this system. Ion-association extraction as heteropolymolybdate-Capriquate could be employed to separate phosphate at several ppm levels from silicate as heteropolymolybdate in the non-aqueous system. The ion-pair of heteropolyacid of As,Si,Ge with Co-5-Cl-PADAP were quantitatively collected on the membrane filters and these ions dissolved in dimethylformamide could be separated by complex-forming equilibria with magnesium in methanol-water mixed solvent system. Less

新迁移系统的开发是研究了，以增强非水，混合和水溶性中毛细管异位液（CTIP）的敏感性和分离。1。新的迁移系统（其终止离子在控制样品物种的有效迁移率上起着重要作用）是通过使用质子溶解性（Dimethylformamide）开发的。在该系统中，终止离子的氯化物充当络合剂，因此注入的重金属离子被转化为氯化物复合物。每个氯化物质的有效迁移率是通过与终止离子形成平衡的络合而控制的，并且可以实现重金属离子的分离。使用氰化物或EDTA作为终止离子，将重金属离子和稀土金属离子在水性和混合溶液中分离。此外，用硼酸盐作为终止离子的迁移系统可能适用于电力中性物种（例如儿茶酚衍生物）的分离。2。 …开发了更多用于将CTIP与预集中的迁移系统相结合，以增强CTIP的敏感性。当使用使用二甲基甲酰胺为溶液的HCIO_4-HCI迁移系统时，可以将提取后重金属离子的DDTC樱桃固定在迁移柱中，并将这些樱桃转换为相应的阴离子氯纤维蛋白质，由Ligand替代反应与Ligand的替代反应，与末端的替代反应。因此，金属作弊可以作为氯化物复合物和分离灵敏度迁移。同样，重金属离子的氧气和二硫酮作弊也可以适用于该系统。可以在非水性系统中以硅质酶为杂聚糖酶的多个ppm水平分离磷酸盐，以分离磷酸盐，以分离磷酸盐。在膜过滤器上定量地收集了AS，Si，GE的异殖酸的离子对，并且这些溶解在二甲基甲酰胺中的离子可以通过在甲基甲基甲基水 - 水混合溶液中的镁与镁的复合形成等效性分离。较少的